1. Repositório Institucional - Universidade Federal de Uberlândia
2. Instituto de Biotecnologia (IBTEC)
3. TCC - Biotecnologia (Patos de Minas)

Tipo de documento:	Trabalho de Conclusão de Curso
Tipo de acceso:	Acesso Aberto
Fecha de embargo:	2019-07-21
Título:	Red onion (Allium cepa L.) methanolic extract increases extracellular nucleotide hydrolysis in rat blood serum
Autor:	Silva, Janayne Luihan
Primer orientador:	Fürstenau, Cristina Ribas
Primer miembro de la banca:	Barbosa, Aulus Estevão Anjos de Deus
Segundo miembro de la banca:	Teixeira, Terezinha Aparecida
Resumen:	Aims: To evaluate the total phenolic content of the red onion (Allium cepa L.) extract, its antioxidant potential and its ability to interfere in extracellular hydrolysis of purine nucleotides in rat blood serum. Materials and methods: Firstly, red onion extract was obtained using methanol as the solvent. Phenolic content and antioxidant capacity of the extract were further analysed. Hydrolysis of ectonucleotidase substrates (AMP, ADP, ATP and p-Nph-5′TMP) were evaluated by colorimetric methods in the presence (or absence) of red onion extract at different concentrations (0, 125, 250, 500 e 1000 µg/mL). Key findings: Total phenolic content of red onion extract was 54.35 ± 0.32 mg GAE 100g-1 sample in the crude extract, which demonstrated antioxidant capacity similar to the standard antioxidant (Trolox) at all tested concentrations (from 125 to 1000 µg/ mL). Correlation between extract total phenolic content and percentage of antioxidant activity was R²=0.9595. Moreover, red onion extract increased ADP hydrolysis at 500 µg/mL (p ≤ 0.05). The same occurred for p-Nph-5'TMP hydrolysis at 500 and 1000 µg/mL (p ≤ 0.05). Significance: Our results confirm that red onion extract presents high levels of antioxidant molecules. Also, the red onion extract may be important to regulate vascular processes, such as decrease platelet aggregation since it increases ADP breakdown, which is an important platelet agonist in the vasculature. The increased ADP hydrolysis may be explained by the fact that ectonucleotidases have their activity favored when the reactive oxygen species (ROS) are removed by antioxidants of red onion extract.
Palabras clave:	Red onion (Allium cepa L.) Phenolic content Antioxidant activity Purinergic signaling Ectonucleotidases
Área (s) del CNPq:	CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::ENZIMOLOGIA
Idioma:	eng
País:	Brasil
Editora:	Universidade Federal de Uberlândia
Cita:	SILVA, Janayne Luihan. Red onion (Allium cepa L.) methanolic extract increases extracellular nucleotide hydrolysis in rat blood serum. 2017. 22 f. Trabalho de Conclusão de Curso (Graduação em Biotecnologia) - Universidade Federal de Uberlândia, Patos de Minas, 2017.
URI:	https://repositorio.ufu.br/handle/123456789/22656
Fecha de defensa:	21-jul-2017
Aparece en las colecciones:	TCC - Biotecnologia (Patos de Minas)

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