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https://repositorio.ufu.br/handle/123456789/22642| Document type: | Trabalho de Conclusão de Curso |
| Access type: | Acesso Aberto |
| Title: | Extraction and characterization of andiroba (Carapa guianensis) oil and its effects on ectonucleotidases of blood serum of rats |
| Author: | Teixeira, Luani de Oliveira |
| First Advisor: | Fürstenau, Cristina Ribas |
| Summary: | Carapa guianensis, popularly known as andiroba, is a tree that grows at Amazon Basin and is used in popular medicine in Brazil. Oil obtained from andiroba seeds is employed to treat inflammation, wound healing, as well as infections and insect repellent. Purinergic signaling helps to control cardiovascular functions which may be related to these pathologies. Thus, the aim of the present study was to evaluate the effects of C. guianensis oil on ectonucleotidase activities from rat blood serum. Oil extraction was performed using hexane as solvent. The procedure yielded 49.11 % of andiroba oil that was used for further characterization and functional assays. Peroxide index resulted in 7,916 ± 0,54 meq peroxide/ 1 Kg sample that signals to high levels of oxidants in the oil. However, low acidity was observed, evidencing that the oil was not in decomposition state. Total phenolic content and antioxidant activity by DPPH method had low results (24.99 mg EAG/ Kg of sample and negative values, respectively), showing that from this methodology, these molecules were not kept in the oil. Besides, hydrolyses of ATP, ADP and AMP in blood serum of rats were not affected by andiroba oil at different concentrations tested (0 µg mL-1, 125 µg mL-1, 250 µg mL-1, 500 µg mL-1 and 1000 µg mL-1). In conclusion, oil extraction from andiroba seeds was good in terms of yield; however, the quality of this andiroba oil was different from others obtained through different methodologies, which importantly impacted the expected effects upon antioxidant capacity of the oil and its effect on nucleotide hydrolysis. |
| Keywords: | Carapa guianensis Andiroba Oil Extraction Oil Characterization Ectonucleotidases Blood Serum |
| Area (s) of CNPq: | CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::ENZIMOLOGIA |
| Language: | eng |
| Country: | Brasil |
| Publisher: | Universidade Federal de Uberlândia |
| Quote: | TEIXEIRA, Luani de Oliveira. Extraction and characterization of andiroba (Carapa guianensis) oil and its effects on ectonucleotidases of blood serum of rats. 2017. 26 f. Trabalho de Conclusão de Curso (Graduação em Biotecnologia) - Universidade Federal de Uberlândia, Patos de Minas, 2017. |
| URI: | https://repositorio.ufu.br/handle/123456789/22642 |
| Date of defense: | 26-Jul-2017 |
| Appears in Collections: | TCC - Biotecnologia (Patos de Minas) |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ExtractionCharacterizationAndiroba.pdf | 549.87 kB | Adobe PDF |  View/Open |
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