Efeito do metabolismo na resposta imune inata do endométrio bovino

Abstract

Bacteria that usually infect the bovine endometrium at postpartum impairs immune defense when exist metabolic stress, leading to endometritis and infertility. The response of the endometrium to bacteria depends on innate immunity, with the recognition of pathogen-associated molecular patterns by stimulating inflammation, characterized by secretion of interleukins (IL) -1p, IL-6 and IL-8. However, animals often fail to eliminate the microbial infection when they have reduced levels of glucose and glutamine, which are sources of energy to the endometrium in the negative energy balance. Continuous inflammation and innate immune response to bacterial infection in the uterus after calving compromise animal welfare. In this study we tested the hypothesis that homeostatic pathways conferred by glucose , glutamine and biotin, link the energy metabolism and innate immunity in the bovine endometrial tissue. The deprivation of glucose reduced the secretion of IL-1P, IL-6 and IL-8 in cultures of endometrial organs challenged with the endotoxin lipopolysaccharide (LPS). Glutamine depletion also reduces the inflammatory activity of the endometrium to LPS. However, glucose and glutamine activities depend on the presence of glycolysis. The vitamin biotin demonstrates anti-inflammatory nature, reducing the production of proinflammatory cytokines in the endometrium. In conclusion, the energy metabolic stress disrupts inflammatory responses to bacterial infections in the endometrium. We provide data on how negative energy balance may be linked to postpartum uterine disease.