Análise da glândula parótida de ratos diabéticos induzidos por estreptozotocina relacionada a alterações de biomarcadores antioxidantes e deposição de colágeno tipo I

Abstract

Acarbose is a competitive inhibitor of intestinal alpha-glycosidases that slows the breakdown of sucrose and starches, thereby reducing glucose and fructose absorption. The aim of this study was to evaluate in parotid gland of diabetic rats the effect of acarbose treatment on antioxidant parameters and deposition of collagen type I. Diabetes was induced by intravenous injection of streptozotocin (STZ). Animals were distributed in four groups (n = 10, each): non-diabetic control (NDM); diabetic (DM); diabetic treated with acarbose at 25 mg/kg (DMA) and non-diabetic control treated with acarbose (NDMA). Changes in enzymatic antioxidant system such as the activity of SOD and GPx enzymes were evaluated in the parotid gland. We found to the DM group high levels of both enzymes that were reverted by acarbose treatment. Tissue damage showed by an increase of TBARS concentration in the parotid gland of the DM group was also reverted in the DMA group. The present study showed that specific stained of the collagen fibers type I in the parotid gland differed from diabetic and non-diabetic rats. These fibers presented high degree of stained in the parotid gland of the diabetic rats when compared to the NDM group. The acarbose treatment was able to reduce in 25% the quantification of these fibers (p < 0.001). These results suggest that the diabetic state influences the collagen type I intensity of parotid gland in rats. In conclusion, one may assume that the acarbose treatment is helpful to prevent the increase in oxidative stress induced by hyperglycemia of DM rats in parotid glands, compared with untreated diabetic rats.