Avaliação da citologia endometrial em cadelas clinicamente saudáveis durante as fases do ciclo estral e com piometra

Abstract

In order to assess the influence of the estrous cycle, bacterial isolation, cystic endometrial hyperplasia ( HEC ) in clinically healthy and without uterine diseases and pyometra in the examination of endometrial cytology bitches, 105 healthy and 33 bitches with pyometra were used. All animals underwent ovariohysterectomy and uteri macroscopically assessed and collected samples for microbiological evaluation, endometrial histology and cytology technique for endometrial brush ( cytobrush ) . The phase of anestrus showed difference in the percentage of neutrophils and endometrial epithelial cells. The bitches endometrial cytology and no histologic changes bacterial isolation in accordance with the phase of the estrous cycle were as follows: a) proestrus (neutrophils 2,05 + 0,64%, eosinophils 0,07 + 0,07%; endometrial epithelial cells 97,87 + 0,63% ), b) estrus (neutrophils 4,55 + 1,76%, eosinophils 0,83 + 0,83, endometrial epithelial cells 94,62 + 1,83) c ) diestrus (neutrophils 7,87 + 4,07%; endometrial epithelial cells 92,13 % + 4,07 ) and d) anestrus (neutrophils 0,24 + 0,24%; endometrial epithelial cells 99,75 + 0,24%). There was an increase in the percentage of neutrophils and endometrial epithelial cells in the proestrus phase of the animals with bacterial isolation. The pyometra interfere with presence of neutrophils increased ( 60,30 + 4,32%), and lymphocytes ( 1,71 + 0,52 %). Neutrophils were more leukocytes found in all cytological evaluations. It was concluded that endometrial cytology is a useful tool in knowledge of cytological changes and the phase of the estrous cycle influence the endometrial cytology. Cystic endometrial hyperplasia and bacterial isolation do not interfere in endometrial cytology. The independent influences pyometra endometrial cytology classification of histological changes, and neutrophils cells more gifts.