Impacto de diferentes períodos de jejum pré-abate na enumeração de micro-organismos indicadores e presença de Salmonella spp. em carcaças de rãs-touro (Lithobates catesbeianus)

Abstract

The bullfrog (Lithobates catesbeianus) is currently the main species used in raniculture in Brazil. The consumption of its meat has been shown a rising market, mainly by the benefits that this product represents for the health of the consumer. For this reason, it has been increasingly important to care for the microbiological contamination of this meat throughout its processing. However, one of the difficulties in obtaining a quality product is the lack of standardization during the slaughter and processing of frog meat. There is no pre-slaughter fasting time specific for the species in Brazilian legislation, and an inadequate fasting may lead to microbiological contamination of the carcass. The objective of this work was to evaluate the impact of different pre-slaughter fasting times on the microbiological contamination of bullfrog carcass in order to recommend an ideal fasting period based on product safety. The first chapter of the dissertation refers to general considerations about the subjects covered in the article, such as the economic aspects of ranching, the slaughter of frogs, the importance of indicator microorganisms and Salmonella spp. in the slaughter process and in public health. In the second chapter, the objective was to assess the impact of different periods of pre-slaughter fasting on the count of indicator microorganisms and the presence of Salmonella spp. in carcasses of bullfrogs slaughtered in an establishment in Uberlândia-MG, Brazil. In addition, the phenotypic and genotypic characterization of Salmonella spp. isolated from the carcasses, being evaluated the presence of virulence genes (invA, lpfA, sefA, sopB, spaN, spvB, orgA, pagC, msgA, iroN and cdtB) by the PCR technique and the sensitivity to ten antimicrobials by the diffusion disk test.