Papel da Variante M235T do Angiotesinogênio e do Polimorfismo de Inserção/Deleção da Enzima Conversora de Angiotensina(ECA) na Doença Arterial Coronariana.

Abstract

Coronary Artery Disease (CAD) is characterized by the formation and accumulation of fatty plaques in the coronary arteries, thus reducing the arrival of oxygen to the heart. Genetic factors appear to be involved in the development of the disease, such as the M235T variant of the angiotensinogen gene (AGT) and the insertion/deletion (I/D) polymorphism of the angiotensin converting enzyme (ACE). The objective of this work was to genotype and analyze the relationship between the M235T variant of AGT and the ACE I/D polymorphism in (CAD). 121 individuals were selected, 74 patients (case) and 47 controls, both undergoing coronary angiography and analysis of conventional risk factors. Peripheral blood samples were obtained at the Hospital de Clínicas of the Federal University of Uberlândia (Consent form attached). and DNA extraction and Polymerase chain reaction (PCR) were carried out to isolate fragments of the ACE I/D polymorphism and the M235T variant of AGT, the latter being subjected to enzymatic restriction, with the enzyme Tth111. After these procedures, agarose gel electrophoresis was performed, stained with ethidium bromide, 3.5% and 2% respectively, for genotyping of the M235T variant and the ACE I/D polymorphism. The results obtained were not statistically significant for the M235T variant and the ACE I/D polymorphism in relation to the occurrence of CAD.