Teste de validação da técnica de bioquímica seca na análise de perfil hepático de amostras de Mus musculus

Abstract

This study evaluated the feasibility of the dry biochemistry technique for analyzing the liver profile in samples of Mus musculus (Balb/c strain) kept at the UFU Rodent Vivarium Network, using the Chemcare Dry Chemistry Analyzer Wondfo®. Parameters such as AST, ALT, albumin and total cholesterol were analyzed and compared with the conventional wet biochemistry technique (BIO-2000IL). The results showed significant inconsistencies, with systematic errors and imprecision, especially for ALT and cholesterol, making it impossible to validate the method without prior recalibration. Despite the operational advantages of dry biochemistry (low sample volume and speed), calibration failed of the equipment compromised the reliability of the data, highlighting the need for technical adjustments for future applications in research with mice.