Caracterização Óptica de Soluções Fluorescentes Inseridas em Salivas Artificiais Para Bioaplicações

Abstract

In this work, the optical characterizations of fluorescent solutions are presented inserted into artificial saliva, with different hydrogen potential values (pH ~ 3.2 – 11.5). Spectroscopy measurements of absorbance, fluorescence, lifetime, and FTIR (Fourier Transform Infrared Spectroscopy) of fluorescent solutions of fluorescein, anthocyanin and carbon nanoparticles inserted into artificial saliva were carried out. The choice of saliva as a research is due to its relevance in various bodily functions, including lubrication, chewing, swallowing, and digestion. Additionally, saliva is capable of reflecting the physiological and pathological state of the body, with their pH serves as an indicator for certain diseases. The purpose of this study is to evaluate fluorescent dyes, such as fluorescein and anthocyanin, as well as carbon dots, as potential pH-dependent biosensors, aiming for disease diagnosis, given the variability of the pH of human saliva in pathological situations.