Avaliação das condições de cultivo de bacteriófagos de Klebsiella quasipneumoniae

Abstract

Bacterial resistance to antibiotics continues to be a critical challenge for public health, highlighting the importance of investigating alternative therapeutic options. This study aimed to evaluate different cultivation conditions of bacteriophages of Klebsiella quasipneumoniae, an opportunistic bacterium that may present strains that are resistant to multiple antibiotics. Four bacteriophages isolated from a sample of the Aricanduva River, in São Paulo, were used, along with the bacterial strain that was used to isolate them. The variables tested included addition of MgSO₄ and/or CaCl₂ to the culture medium and incubation at temperatures of 30 °C and 37 °C. The results indicated that the different conditions did not significantly impact replication of the phages. Optimization of the phage cultivation, in order to increase its production, is essential for the potential use of bacteriophages as a viable alternative for controlling K. quasipneumoniae and K. pneumoniae infections.