Efeitos da hidroxiapatita (CaHA) e do ácido poli-lático (PDLA) na bioestimulação de fibroblastos: uma análise metabolômica, citotóxica e de citocinas sob a influência da terapia com ozônio

Abstract

The study investigates the effects of calcium hydroxyapatite (CaHA) and poly(DL-lactic acid) (PDLA) on human foreskin fibroblasts (HFF-1), particularly in the context of ozone (O3) therapy. The cell viability assay showed no cytotoxic effects of the bio-stimulators under the tested conditions, with optimal concentrations determined at 300 μg/ml, 100 μg/ml, and 30 μg/ml for CaHA, and 300 μg/ml, 100 μg/ml, and 30 μg/ml for PDLA. Ozone concentrations tested were 5 μg/ml, 10 μg/ml, and 15 μg/ml. The Scratch assay revealed that CaHA and PDLA, both alone and combined with ozone, promoted cell migration and proliferation. Metabolomic analysis indicated significant activation of inflammatory and collagen synthesis pathways in HFF-1 cells treated with CaHA, PDLA, and ozone. Elevated levels of metabolites such as D-Pantothenic acid and N-(1-Deoxy-1-fructosyl)leucine suggest enhanced biosynthetic activity and oxidative stress responses, contributing to collagen production and tissue renewal. The study concludes that CaHA and PDLA, particularly when combined with ozone, have potential therapeutic applications in enhancing skin regeneration and combating signs of aging.