Uso do soro de indivíduos com obesidade no estudo in vitro do núcleo celular em linhagem de glioblastoma humano

Abstract

Glioblastoma is characterized as one of the most aggressive malignant neoplasms with an average survival rate after diagnosis of 12 to 15 months. Currently, cases of obesity have reached pandemic levels, and it is a risk factor for various types of cancer, and may contribute to the development, progression and support of the tumor environment through various pathways. The study of cell nucleus architecture has emerged as a promising field for understanding the mechanisms underlying malignant neoplasms. Therefore, this study analyzed nuclear alterations in human glioblastoma cell cultures when exposed to serum from obese people. To this end, the serum of 12 volunteers, 6 of whom were obese and 6 of whom were not, was collected and used to assess genetic instability using the micronucleus assay and to assess chromatin organization using the fluorochrome DAPI. In addition, the SF-36 quality of life questionnaire and the Baecke questionnaire were used to characterize the profile of the volunteers. There was no statistically significant difference between the obese and non-obese groups in terms of the answers to the questionnaires. However, the cells treated with serum from the obese group showed a higher frequency of micronuclei, a larger nuclear area and a lower presence of heterochromatin. Further studies are needed to identify specific components that may lead to these changes, but these results indicate that obesity favors genetic instability and induces changes in chromatin organization, which may influence a cascade of events favourable to the progression of glioblastoma