1. Repositório Institucional - Universidade Federal de Uberlândia
  2. Instituto de Ciências Biomédicas (ICBIM)
  3. TCC - Ciências Biomédicas
Please use this identifier to cite or link to this item: https://repositorio.ufu.br/handle/123456789/36390
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dc.creatorTanaka, Camila Ayumi-
dc.date.accessioned2022-11-16T13:17:30Z-
dc.date.available2022-11-16T13:17:30Z-
dc.date.issued2022-08-12-
dc.identifier.citationTANAKA, Camila Ayumi. Infection of mice with a Brazilian clinical isolate of Mayaro virus results in mild disease. 2022. 14 f. Trabalho de Conclusão de Curso (Graduação em Biomedicina) – Universidade Federal de Uberlândia, Uberlândia, 2022.pt_BR
dc.identifier.urihttps://repositorio.ufu.br/handle/123456789/36390-
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Uberlândiapt_BR
dc.rightsAcesso Embargadopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectMayaropt_BR
dc.subjectExperimental modelpt_BR
dc.subjectEmergentpt_BR
dc.subjectPublic healthpt_BR
dc.titleInfection of mice with a Brazilian clinical isolate of Mayaro virus results in mild diseasept_BR
dc.typeTrabalho de Conclusão de Cursopt_BR
dc.contributor.advisor-co1Rosa, Rafael Borges-
dc.contributor.advisor-co1Latteshttp://lattes.cnpq.br/9326917459081024pt_BR
dc.contributor.advisor1Mineo, Tiago Wilson Patriarca-
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/4014578382806189pt_BR
dc.contributor.referee1Silva, Murilo Vieira da-
dc.contributor.referee1Latteshttp://lattes.cnpq.br/8828671886211923pt_BR
dc.contributor.referee2Santos, Igor de Andrade-
dc.contributor.referee2Latteshttp://lattes.cnpq.br/6589573434199212pt_BR
dc.description.degreenameTrabalho de Conclusão de Curso (Graduação)pt_BR
dc.description.resumoBackground: Mayaro virus (MAYV) is the etiologic agent of Mayaro fever and is currently considered an emergent and neglected disease. Several factors indicate the possibility of urbanization of this virus that may configure the disease as a major public health problem. Cases of Mayaro fever have been confirmed in different Brazilian states, which supports the possible adaptation of the vector to the urban environment. The characterization of the Brazilian isolate of Mayaro virus (MT/SINOP/210/2011) in a murine model may help in the elucidation of disease mechanisms, pathogenesis, and contribute to advancing the development of treatments and prophylactic measures. Methods: IFN-α/β receptor-deficient (B6.129S2-Ifnar1) or congenital controls (129Sv/Ev) female and male mice at 21 days of age were inoculated via footpad with 105 TCID50 of MAYV or PBS in a volume of 20 μL. Survival, body weight, and paw edema were assessed daily. A clinical score was determined using a numerical value and employed in phenotypic disease monitoring. Three and six days after infection, blood, joints, and muscles were collected for hematology and histopathology evaluation, respectively. Findings: Knockout mice were susceptible to infection, developing clinical signs of the disease, which was not observed in animals with an intact immune system. There were no changes in the animals' body weights and the score evaluation demonstrated that infected male mice showed more pronounced clinical signs compared to females, such as diarrhea, purulent conjunctivitis, and plantar cushion edema. MAYV infection in knockout mice led to a low lethality rate. Histopathological analysis revealed that there was a greater inflammatory response in the acute phase of the disease in infected knockout animals compared to wild-type animals. Mild edema and vasculitis were seen in both knockout and wild-type animals. Finally, there was an intense cellular infiltrate of lymphocytes in the acute phase of the disease in knockout and wild-type. Interpretation: The infected knockout animals showed mild clinical signs of the disease. The infection had a benign course and most were able to control the infection. The signs observed lasted only a few days, coinciding with the period of the acute phase of Mayaro fever in humans, therefore, the disease manifests itself mildly in humans and mice, demonstrating that the circulating strain MT/SINOP/210/2011 has low virulence and the experimental model is compatible with the course of infection in human patients.pt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.courseBiomedicinapt_BR
dc.sizeorduration14pt_BR
dc.subject.cnpqCNPQ::CIENCIAS DA SAUDEpt_BR
dc.embargo.termsO presente estudo será submetido para publicação em periódico científico.pt_BR
dc.description.embargo2024-08-12-
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