Avaliação do potencial osteocondutor e osteoindutor de scaffolds 3d-nanofibrilares de sílica: estudo in vivo

Abstract

Bone defects are frequently related to the clinic practice. Synthetic substitutes and materials for grafts from different production and origins have been used to promote and potentialize bone formation during the tissue repair. The purpose of this study was the silica 3D-nanofibrous scaffolds bioactivity (bioactive glass) evaluation in vivo, produced by the SBS (Solution Blow Spinning) technique for bone regeneration. The nanofibers utilized in this study were obtained by SBS with the composition of 65% SiO2, 31% CaO and 4% P2O5 mol, in two macroscopic conformations: body or fiber. To evaluate the biological effect, bone defects were created on the femur of 15 Wistar male rats. The detects were filled with blood, Body Bioactive Glass or Fiber Bioactive Glass, and the animals were sacrificed after 14 or 28 days. The femur were collected and processed to obtain the histological slides. A histological descriptive analysis of the bone repair was made, associated to the biomaterial. Furthermore, the bone neoformation and the biomaterial remaining particles were quantified for the histomorphometric analysis. The qualitative and quantitative analysis revealed a quality bone formation and an activity of remodeling in grafted sites with bioactive Glass in comparison to the bioactivity and biocompatibility of the nanofibrous scaffolds fabricated through the SBS technique.