Estudo do efeito da ozonioterapia através de soro ozonizado em peritonites induzidas experimentalmente em ratos Wistar

Abstract

Ozone is a natural gas molecule composed of three oxygen atoms in a dynamically unstable structure due to the presence of the mesomeric effect. It is one of the most important gases in the stratosphere because of its ability to filter ultraviolet rays and thus ensure the maintenance of biological equilibrium in the biosphere. Since World War I it has been used for medical treatment as it possesses antibacterial, hemodynamic and anti-inflammatory properties. However, studies to understand the effect of ozone on peritonitis and to propose safe ways to use the gas in cases of peritonitis are still lacking. The present experimental study aimed to evaluate the action of ozone serum in the treatment of peritonitis in Wistar rats. For this, the induction of peritonitis was performed by intraperitoneal inoculation of fecal suspension in these animals. These were divided into 3 groups and each group received a different treatment: intraperitoneal saline, intraperitoneal ozonated saline and control (no treatment). Analysis of cavity fluid was performed in two distinct moments: 24 hours after the inoculation, which confirmed that all the animals were in the clinical condition of peritonitis, and after 7 days into the study, and by comparing this data we arrived at results that the group which was not receiving the treatment (control) had a worsening in the inflammatory condition with a 7% increase in inflammatory cells, the group that received intraperitoneal Ringer Lactate solution had an improvement with a 67% decrease in inflammatory cells, and finally the group that received intraperitoneal ozonized Ringer Lactate solution had a further significant improvement coming to a reduction of 84.5% of inflammatory cells, reaching a value that, according to the reference used, does not fit into the clinical condition of peritonitis. It was concluded that the use of intraperitoneal ozonized serum was effective as an experimental treatment in induced peritonitis in Wistar rats.