Susceptibilidade aos antimicrobianos de Staphylococcus pseudintermedius isolados de cães e seus tutores

Abstract

ABSTRACT

Antimicrobial resistance is considered a worldwide public health problem. This study aimed to evaluate the susceptibility of strains of 91 Sthapylococcus pseudintermedius strains isolated from dogs with otitis (4) and recurrent pyoderma (43) and their guardians (44) to the antimicrobials oxacillin, gentamicin and cefovecin by two diagnostic methods, the minimum inhibitory concentration (CIM) and the disk diffusion test, and check if the results obtained in the diffusion test can be associated with the results of the CIM (gold standard). The strains originated from 50 dogs and their guardians. The results were used to build resistance profiles for isolates from dogs and humans. Five antimicrobial resistance profiles were observed, with decreasing resistance of S. pseudintermedius to oxacillin, cefovecin and gentamicin, the latter being the most suitable for the treatment of recurrent pyoderma and otitis in dogs. The profile with strains sensitive to the three antimicrobials was the most prevalent and comprised 53.88% (49/91) of the strains. We observed a weak association between resistance to oxacillin and cefovecin (r = 0.392) by strains of S. pseudintermedius isolated from animals and humans, characterizing that the phenotypic resistance of one does not reflect in the other. It was not possible to establish the association for gentamicin. The evaluation of the disk diffusion test to determine resistance to oxacillin and cefovecin demonstrated that the method has low sensitivity to determine resistant S. pseudintermedius when compared to the gold standard, MIC. Thus, even though it is considered a low-cost and easy method to perform, its use is not indicated to establish the resistance of S. pseudintermedius to these two antimicrobials.