Efeito de frações de polifenóis de Annona crassiflora Mart. E nanocompósito de ZnO7Ag, sobre o fechamento de feridas cutâneas e angiogênese.

Abstract

Among the possible recently researched technologies that can be applied in wound care are nanomaterials. Moreover, research using plant compounds, due to their polyphenol content, is promising. Thus, this study evaluated the healing effect of topical use of polyphenol fractions of the araticum and zinc oxide (ZnO7Ag) nanocomposite. The formulation was incorporated into the vehicle (lanolin and vaseline) and used for daily treatment in induced wounds in Balb / C mice. Five groups were evaluated: untreated control (CO s/trat.), Injured animals and not treated, vehicle control (CO / vei.), Injured animals and treated with the vehicle only, treated with fractions of araticum bark polyphenols (ART), treated with nanocomposite (ZnO7Ag) and treated with nanocomposite polyphenols (ART + ZnO7Ag). The closure of the wounds was measured with a digital caliper and evaluated at times 0, 3, 7, 14 days of treatment. Angiogenesis was evaluated by blood vessel count in Gomori-stained photomicrographs at times 3, 7, 14, 21 days. After 7 days of treatment, ZnO7Ag demonstrated the best results for wound closure, and wound contraction continued to increase after 14 and 21 days, which is the best effective wound contraction treatment. After 7 days of treatment, ART + ZnO7Ag showed higher angiogenic activity and was increased again after 14 days, proving to be the most effective treatment in increasing blood vessels in the lesion. It was concluded that treatment with ZnO7Ag led to the optimization of wound repair, demonstrating an improvement in the time required for closure. And polyphenol enriched fractions of Annona crassiflora Mart. associated with nanocomposite (ART + ZnO7Ag) influenced the increase of angiogenesis, which contributes to nutrient and oxygen availability.