Efeito de extratos metanólicos de Calliandra dysantha Benth. (Fabacea) sobre o biofilme de Staphylococcus aureus

Abstract

Bacterial biofilms are communities of microorganisms surrounded by an extracellular matrix, produced by themselves, conferring protection against the external environment and resistance to antimicrobial compounds. Although biofilms are found in nature, acting beneficially as decontaminating agents of aquatic environments, they can cause damage to humans when they are formed on surfaces of industrial equipment and medical devices. Among the pathogenic bacteria that produce biofilm, Staphylococcus aureus is one of the main microorganisms related to infections caused by antimicrobial resistant bacteria. Thus, the search for new active compounds capable of treating infections caused by S. aureus, as well as fighting its biofilm is necessary. Previous work, carried out at the UFU Microbiology Laboratory, Patos de Minas campus, demonstrated the antimicrobial activity of methanolic extracts prepared from different organs of Calliandra dysantha Benth. against S. aureus. However, there are no studies in the literature demonstrating the effect of this plant on S. aureus biofilm. Thus, the present study aimed to evaluate the effect of different concentrations of methanolic extracts prepared from C. dysantha Benth stem, leaf and flower against S. aureus biofilm. Flower and stem extracts showed antibiofilm activity with lower cell viability of 63.1% and higher viability of 86.4% for flower and stem of 69.3% and 90.4% respectively, while leaf extract presented pro biofilm activity with minimum and maximum cell viability of 188.0% and 249.6%. These effects may be related to the presence of different compounds such as a CasuL lectin and ellagic acid, respectively.