Identificação de paternidade em progênies de Paspalum l. via marcadores moleculares microssatélites

Abstract

Belonging to the grass family, the genus Paspalum L. has more than 350 species, many of which have great forage potential. Embrapa Pecuária Sudeste carries out research with the genus, and most species belong to the informal group Plicatula. Most of this group is apomitic tetraploid (2n = 4x = 40), and it is difficult to find sexual diploids. Embrapa, in its genetic breeding program, polyploidized sex diploid genotypes (BGP 281 - P. lenticulare; BGP 272 - P. rojasii and BGP 380 - P. compressifolium) that were planted in the field for possibly hybrid seed production. the observation of self-compatibility in sexual genotypes of this group. The present work aimed to recognize the hybrid progenies of the crossings between the three accessions, by identifying paternity via microsatellite molecular markers. Eight microsatellite markers known from the literature were used, obtaining results from six markers. Forty-four progeny samples were collected, plus three parent samples. It was possible to identify 36 hybrid progenies among all samples, most of the crossing between accessions BGP (380) and BGP (281).