Diferenças de enantiômeros do Citalopram na indução de mutação e recombinação em células de asa de Drosophila melanogaster

Abstract

The main goal of this research was to evaluate the mutagenic and recombinogenic effect of Citalopram and Escitalopram in somatic cells of Drosophila melanogaster and verify the influence of CYP450 complex enzymes in the induction of mutation and homologous recombination. Flies from the Standard (ST) and High Bioactivation (HB) crosses were used for the Somatic Mutation and Recombination Test (SMART). Third instar larvae of D. melanogaster obtained from both crosses (ST and HB) were fed chronically with the antidepressants drugs Citalopram or Escitalopram (0,3125 to 10 mg/mL). Both drugs were toxic in higher concentrations. The results obtained from the crosses indicated that Citalopram was mutagenic and recombinogenic only at the highest concentration tested in ST. However, it did not show any mutagenic effect when highly metabolized via CYP450 enzymes. Escitalopram was mutagenic and mainly recombinogenic in all ST treatments, but it was completely mutagenic in all HB treatments. More studies need to be done to better evaluate the DNA damage caused by these drugs.