Análise do comportamento de células tumorais pulmonares A549 em contato com scaffold biológico

Abstract

In order to investigate the association of cells with a collagen scaffold manufactured by our group, viability assays were done to evaluate the cytotoxic potential of the scaffold over A49 cells. In addition, we investigated the morphology of cells in contact with the scaffold. To reach our goals, the Alamar Blue viability assay was performed and cell morphology was assessed using micrographs. Our results show that cells did not have a significant difference of viability after 24 and 72 hours of contact with the scaffold. However, after 48 hours of contact, a moderate interference in the cell viability was noticed, compared to its control. Relevant morphological changes were observed on cells grown on scaffolds, compared to the ones cultivated on monolayer culture. Our results show that the collagen gel evaluated on this study is biocompatible with cell line A549 and that the morphologic differences found are consistent with those described on the literature.