Influência dos métodos de seleção espermática (swim-up, gradiente de Percoll® e washing), na Fertilização in vitro, associado a dinâmica do desenvolvimento embrionário em bovinos

Abstract

During in vitro fertilization (IVF), spermatozoa need to undergo sperm selection to become capable of fertilizing oocytes. In order to obtain efficiency in IVF, methods of sperm selection were developed. In this sense, the objective of this study was to analyze the correlation of sperm selection with daily growth rate and embryo quality during development. IFV routines were performed comparing Percoll, Swim-up and Washing methods, analyzing the rates of cleavage and blastocysts, and photographing the embryos during in vitro culture for daily growth analysis, number of blastomeres and quality of the same. A greater (P <0.05) cleavage rate was observed in the Swim-up group compared to Washing, and in the Percoll group a greater (P <0.05) blastocyst rate was obtained in relation to the Swim-up. The Washing group resulted in embryos with quality and number of lower blastomeres (P <0.05) and higher (P <0.05) percentage of degenerate embryos than the other groups. The number of blastomers and the growth rate were positively associated with embryo quality, and in addition, embryos with moderate and rapid growth are more likely to reach the blastocyst stage than those with null growth. Finally, we conclude that the Washing method adversely affected embryonic development and is not a recommended screening method for IVF. In addition, embryonic quality and rate of growth rate can be used as predictors for good in vitro production (IVEP) results.