Atividade farmacológica anti-inflamatória do extrato aquoso de Eugenia dysenterica (Myrtaceae) DC em modelo experimental in vivo

Abstract

Eugenia dysenterica, popularly known as cagaita, is a fruit species native to the Brazilian Cerrado and belong to the Myrtaceae family. Phenolic compounds in this species have been related to the antioxidant action of cagaita. However, there are no studies evaluating the antiinflammatory potential of E. dysenterica. Thus, this study proposed to investigate the possible anti-inflammatory effects of the aqueous extract of the leaves of E. dysenterica in experimental model in vivo. The aqueous extract was prepared from the dried and crushed leaves at a ratio of 20% (w / v), followed by freezing and lyophilization. For the evaluation of anti-inflammatory pharmacological effects, initially the carrageenan-induced paw edema method was standardized. BALB/c mice were orally treated with 0.5% carboxymethylcellulose or indomethacin (10 mg / kg). After 60 minutes, the animals received an injection inside the paw of carrageenan (30 μL at 0.5% or 1.0%) in the right hind paw and saline solution (NaCl 0.9%, 30 μL) in the left hind paw (control). Paw volume determination was performed on a plethysmometer. Measurements of edema were recorded before carrageenan injection and after 0.25, 0.5, 1, 2, 4, and 6 hours. Edema was expressed as the increase in volume (mL) of the paw treated with CGN compared to the control paw at each time interval. Euthanasia was then performed by cervical displacement of the animals and the right and left hind paws were sectioned at the tibio-tarsal joint with a scalpel blade and placed in falcon tubes containing 10% formalin for fixation. Afterwards, the fingers were sectioned and made longitudinal cut for inclusion in paraffin, staining of the blades and evaluation of inflammatory infiltrate in the plantar muscle. After the standardization, the experiments with the concentrations of 100 mg/kg, 550 mg/kg and 1000 mg/kg of Eugenia dysenterica aqueous extract administered by gavage were performed. The positive control group was administered orally, indomethacin (10mg / kg); negative control was administered 0.5% CBX. After sixty minutes, the animals received an injection in one of the hind paws of 0.5% carrageenan in the right paw and 0.9% NaCl in the left paw and the paw volume was measured in plethysmometer before the injection of carrageenan and after 1, 2, 3, 4, 5 and 6 hours. Statistical evaluation of the data obtained showed that the aqueous extract of Eugenia dysenterica was not effective in reducing paw edema induced by carrageenan in this experimental model.