Cerâmicas de fosfato de cálcio em contato com células mononucleares humanas: estudo in vitro

Abstract

The bioceramics has been widely used in Dentistry for fillings of bone defects. With the objective to evaluate the cell viability was performed an in vitro study where we used three types of bioceramics. The cytotoxic effect of the materials was evaluated in two different ways: peripheral blood mononuclear cells of healthy adults tagged for 24 hours in direct contact with synthetic hydroxyapatite (HA), beta tricalcium phosphate synthetic (BTCP), an association of both materials composed of 60% synthetic hydroxyapatite and 40% beta tricalcium phosphate synthetic (HABTCP) and by serial dilution. After 24 hours, the plates were test cell viability by MTT-Formazan Product, with reading at 570 nm. For statistical analysis, non-parametric test was applied Kruskall-wallis Test for multiple analyzes (α=0.05). In 24 hours, no significant differences were found in the levels of absorbance of the mononuclear cells grown in contact with the three materials in analysis (p>0.05). In the tests of dilution also no significant differences were found when the materials were compared with each other or even among themselves in various dilutions. None of the materials was significantly different from the positive control. It appears that the biomaterials evaluated did not interfere with the cellular viability.