Scaffolds Poliméricos por Salt Leaching de Poli (Metacrilato de Metila) com Porfirina para Aplicações Biotecnológicas.

Abstract

This project aim the development of polymers films and scaffolds with the incorporation of porphyrin molecules that has biological activities for application in biotechnology. The polymer scaffolds are highly porous three-dimensional structures that can be used in cell/tissue growth, drug delivery, molecule carries that promote cell death etc. The photophysical properties of free base porphyrin monomer molecules (5,10,15,20-Tetrakis(4 methoxyphenyl)-21H,23H porphine) in chloroform was investigated by absorption measurements, where the presence of two sub-bands in B band (Soret) localized between 400-420 nm was observed as well as a solder in Q band and four sub-bands localized between 480-650 nm. As the confectioned film and scaffolds have different concentrations of porphyrin molecules, present the changes as the concentration increases in the absorption, fluorescence emission and excited fluorescence emission measurements in the solutions. The absorption measurements show red shift, where the fluorescence emission and excited emission we see changes in the line form of the solution with higher concentration, showing possible presence of aggregates. Next we made the films and scaffolds by adding the porphyrin molecules in different concentration in polymeric Poly (methyl methacrylate) (PMMA) solutions. The films was made by casting forming structure of guest-host type and the scaffolds by salt leaching forming porous structures. The MEV and AFM measurements of the samples allow the qualitative analysis (visual) and quantitative analysis by first and second order statistic. Besides, we studied the photophysical properties of films and scaffolds of PMMA/Porphyrin. The UV-Vis absorption, fluorescence and excited fluorescence emission measurement also show red shift for the higher concentrations, revealing that even in solid structures the aggregation is present and are probably coexistence of H and J type.