Correlações entre estabilidade de membrana de eritrócitos e variáveis oxidativas, inflamatórias e antropométricas em adolescentes obesos

Abstract

[Introduction] Obesity is considered an important public health problem today and has been gaining prominence in the world epidemiological scenario. It is related to a systemic low-grade inflammation. In addition, cell damage related to free radicals is accentuated in obese individuals and predisposes to comorbidities. The stability of a biological membrane depends on genetic, food and lifestyle influences, and seems to suffer alterations due to obesity and oxidative stress. [Objective] This study aimed to investigate the existence of correlations between osmotic stability of erythrocytes and inflammatory, anthropometric, and oxidative stress parameters in obese adolescents. [Sample] The sample consisted of 32 adolescents aged 14 to 19 years. [Methods] The anthropometric parameters used were body mass (BM), body mass index (BMI) and waist circumference (WC). Determination of erythrocyte membrane stability was performed using the erythrocyte osmotic fragility (EOF) test. Oxidative stress was evaluated by means of ferric reducing antioxidant power (FRAP), thiobarbituric acid reactive substances (TBARS), catalase (CAT) and superoxide dismutase (SOD) activities, and sodium nitrite (NaNO2) content. The inflammatory state was verified by measuring C-reactive protein (CRP), adiponectin and tumor necrosis factor alpha (TNF-α). [Results] Positive correlations were found between body composition parameters (BM, BMI and WC) and membrane stability variables (H0, H50 and H100). Positive correlations were also found between the serum levels of SOD and NaNO2 and erythrocyte osmotic stability indices (dX/H50 and dX/Amin, respectively) [Conclusions] Correlation analyzes showed that erythrocyte membrane stability is sensitive to changes in body composition and serum levels of SOD and NaNO2 in obese adolescents.