Síntese, caracterização e aplicação do poli (ácido 3-Hidroxifenilacético) no desenvolvimento de biossensor para detecção de marcador cardíaco

Abstract

In this work was realized characterization studies of a new material, poly (3- hidroxyphenylacetic acid) and its application for the construction of an amperometric immunosensor for detection of Acute Myocardial Infarction (AMI). Initially, it was carried out the electropolymerization of 3-hidroxyphenylacetic acid at three different pH's (0.0, 6.5 and 12.0) which could be assessed, electrochemically, the relationship between the behavior of the polymer and the reaction s pH. It was found that in the acid solutions (pH 0.0) the formation of electrochemically active material is more evident. Moreover, using electrochemical techniques, were carried out investigations on the structure of the polymeric material by using cationic and anionic probes. Monomer and polymer were characterized by infrared spectroscopy (FTIR), Ultra-Violet (UV/Vis.) and fluorescence; thermal analysis (DTA and TGA) and structural analysis (XDR). These studies were extremely important to highlight the main differences between the starting material (monomer) and electropolymerized material (polymer), and in particular assess the main characteristics of the polymer in order to enable its use as a platform to the proposed immunosensor. Studies of the immunosensor were conducted using two substances that acted as indicators of the reaction between the specific antibody for the AMI (anti-troponin T) and specific antigen for AMI (troponin T), hexaaminruthenium chloride and ferro/ferricyanide potassium. The results showed that the hexaaminruthenium chloride showed the best performance to indicate the formation of antibody-antigen complex wich occurs in the AMI.