Reparo em osso irradiado utilizando enxerto ósseo autógeno associado a plasma rico em plaquetas: análise histomorfométrica em coelhos

Abstract

The aim of this study was to observe the repair in irradiated bone using autogenous bone graft associated with platelet-rich plasma in rabbit model. Twelve New Zealand White rabbits were divided into two equal groups: irradiated (I) and non-irradiated (II), which were divided into subgroups: control (left tibiae) and PRP (right tibiae). The irradiated group received a single dose of 15 Gy in the tibiae bone. After forty-five days, in groups I and II, were created lesions in the tibiae, with trephine of 5 mm of diameter. The lesions were filled with autogenous bone grafts with PRP (test group) or without PRP (control group). After four weeks, the animals were sacrificed and the grafted regions were removed, fixed in formalin in PBS, demineralized in EDTA and embedded in paraffin. The histological images were captured at 4X magnification and submitted to the process of Targeting Interactive, as recommended by Oliveira et al. (2006). The region of bone matrix was called Region of Interest (ROI) and the percentage of matrix by area was obtained and analyzed through algorithms developed in the programming environment Scilab. The data were processed using the tests Kolmogrorov-Smirnov and paired ttest student and not paired. The statistical analysis of irradiated and nonirradiated groups showed no significant difference between test and control groups. Also, there s no significant difference between groups irradiated and non-irradiated. According to data obtained, the use of autogenous bone grafts associated with the PRP did not contribute significantly in the repair of irradiated bone.