Frações antigênicas de metacestódeos de Taenia saginata obtidas por cromatografia de troca iônica no imunodiagnóstico da neurocisticercose humana

Abstract

Neurocysticercosis (NC), is common infection of the human central nervous system, diagnosis is established by the combined analysis of clinical data, neuroimaging (computerized tomography and magnetic resonance), immunological and epidemiological data. Serological tests as enzyme linked immunosorbent assay (ELISA) and immunoblotting to detect specific antibodies against metacestodes of T. solium in serum samples or cerebrospinal fluid may provide a viable tool for the diagnosis of NC. The use of heterologous antigen from Taenia saginata metacestodes for NC immunodiagnosis is important where the collection of T. solium metacestodes for the preparation of homologous antigen is difficult. The aim of this study was to fractionate and partially characterize fractions obtained from the total saline extract (SE) of T. saginata metacestodes after ion-exchange procedure in carboxymethyl sepharose (CM) and diethylaminoethyl sepharose (DEAE) resins, as a source of antigenic markers applicable in the immunodiagnosis of NC. For IgG detection by ELISA and Immunoblotting, 140 serum samples were analyzed: 45 from patients with NC (G1), 50 from patients with other parasitic infections (G2) and 45 from healthy individuals. Sensitivity (Se), specificity (Sp), area under curve (AUC) and likelihood ratios (LR) were calculated. CMS2 and DEAES2 fractions provided high diagnostic values (Se 88.8% and 93.4%; Sp 93.7% and 92.6%; AUC 0.965 and 0.987, LR+ 14.07 and 12.67; LR− 0.11 and 0.07, respectively). In conclusion CMS2 and DEAES2 fractions are important sources of specific peptides, with high efficiency to diagnose NC.