Publicação: Promising nanostructured lipid carriers-based essential oils with activity against strains of Campylobacter ssp. isolated from chicken carcasses
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CNPQ::CIENCIAS DA SAUDE, CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA MEDICA
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Ribeiro, Ligia Nunes de Moraes
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Campylobacter is a virulent Gram-negative bacteria genus present mainly in poultry and 13 broilers intestines. The indiscriminate use of traditional antibiotics, such as 14 ciprofloxacin and other fluoroquinolones, led to drug resistance of these virulent 15 pathogens remaining a public health issue. Thus, it is necessary to develop new 16 therapies that are more efficient and less toxic, which is mandatory for use in biological 17 applications. Essential oils (EO) are lipids arising from the secondary metabolism of 18 vegetables, besides showing complex and biologically active structures, its clinical use 19 is limited. Nanotechnology seems to be an alternative to increase bioavailability, 20 stability and biocompatibility, decreasing the photodegradation and toxicity of EO. In 21 this work, 14 EO were evaluated using the diffusion disk test, and it was selected 5 EO 22 with the best anti-campylobacter activity. Then, these liquid lipids were used as active 23 and structural compounds in the composition of nanostructured lipid carriers (NLC). 24 Such resultant formulations were tested through the minimum inhibitory concentration 25 (MIC), in order to find the lowest concentration able to inhibit the Campylobacter ssp. 26 growth. These systems were also monitored in terms of particle size (nm), 27 polydispersity index (PDI) and Zeta potential (mV), confirming its physicochemical 28 stability for 210 days at 25 °C. Morphological analyses through FE-SEM elucidated 29 spherical shape with well-delimited contours of nanoparticles. Then, the best NLC was 30 tested regarding the nanotoxicity, through the chicken embryo model. In relevance to 31 the above results, NLC-based geranium EO was the most promising and safe system for 32 the control and treatment of multidrug-resistant strains of Campylobacter.
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Geranial, Cinnamon, Nanobiotechnology, Bioavailability, Toxicity
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PIRES, Henrique Machado. Promising nanostructured lipid carriers-based essential oils with activity
against strains of Campylobacter ssp. isolated from chicken carcasses. 2023. 16 f. Trabalho de Conclusão de Curso (Graduação em Biotecnologia) – Universidade Federal de Uberlândia, Uberlândia, 2023.
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