Desenvolvimento de um imunossensor para detecção de proteína c reativa

Abstract

This paper describes the development of immunosensor for C reactive protein (CRP), based in direct and indirect electrochemical detections. The anti-CRP probe was grafted to the platform sensitized with poly(3- aminothiophenol), and the interaction of CRP target with the immunosensor was carried directly or indirectly, using 4-aminoantipyrine (4-APP) as indicator. The immunosensor presents a linear response in the range from 75 ng·mL−1 to 150 μg·mL−1 and detection limit of 75ng·mL−1 (N=3). The biosensor specificity determined in the presence of non-specific target is also presented in this paper. Assays realized in real samples indicate that the immunosensor discriminates the serum samples from healthyand sick patients with efficiency.In addition, through atomic force microscopy, it was possible to observe differences between the immunosensor surface in the absence or presence of the CRP target