Publicação:
Isolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose Effect

Página do item simplificado
dc.contributor.advisor1Santiago, Fernanda Maria
dc.contributor.advisor1Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4769119A0pt_BR
dc.contributor.referee1Naves, Karinne Spirandelli Carvalho
dc.contributor.referee1Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4762339J3pt_BR
dc.contributor.referee2Junior, Jair Pereira da Cunha
dc.contributor.referee2Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795802Y5pt_BR
dc.creatorMota, Danielly Christine Adriani Maia
dc.creator.Latteshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K8030220Y2pt_BR
dc.date.accessioned2018-01-24T18:14:06Z
dc.date.available2018-01-24T18:14:06Z
dc.date.issued2017-12-20
dc.description.degreenameTrabalho de Conclusão de Curso (Graduação)pt_BR
dc.description.resumoMorinda citrifolia, known as Noni, is used in folk medicine with several therapeutic applications. The present study investigated the enzymatic and biological characteristics of the papain-like cysteine proteases (PLCP) from M. citrifolia seed. Its enzyme was purified in two-step by DEAE-Sephacel and Phenyl-Sepharose CL-4B (10 × 0.6 cm) columns. PLCP enzyme demonstrated an apparent molecular mass of 51 kDa on SDS-PAGE 14%. The proteolytic activity in the presence of fibrinogen with the PLPC of M. citrifolia demonstrated total hydrolysis of the fibrinogen chains, occurring in the concentration of 20 μg; with the optimum pH of 7.0; temperatures range from room temperature to 50°C; better activity with calcium ions and the effect of inhibition appeared in the presence of Leupeptin. There was no platelet aggregation or coagulation activity. PLCP enzyme was not cytotoxic for cells such as Human Foreskin Fibroblasts (HFF) and murine embryo fibroblasts strain NIH/Swiss (NIH/3T3) macrophages and showed to be effective when tested directly against Toxoplasma gondii infection in vitro. In that sense, this study indicates perspectives for pharmacological applications of M. citrifolia enzymes.pt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.identifier.citationMOTA, Danielly Christine Adriani Maia. Isolation and characterization of papain-like cysteine proteinases from Morinda citrifolia seed with anti-toxoplasmose effect. 2017. 23 f. Trabalho de Conclusão de Curso (Graduação em Biomedicina) - Faculdade de Ciências Biomédicas, Universidade Federal de Uberlândia. Uberlândia, 2017.pt_BR
dc.identifier.urihttps://repositorio.ufu.br/handle/123456789/20358
dc.languageengpt_BR
dc.publisherUniversidade Federal de Uberlândiapt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.courseBiomedicinapt_BR
dc.rightsAcesso Abertopt_BR
dc.sizeorduration23pt_BR
dc.subjectMorinda citrifoliapt_BR
dc.subjectCysteine proteasept_BR
dc.subjectproteolytic activitypt_BR
dc.subjectToxoplasma gondiipt_BR
dc.subject.cnpqCNPQ::CIENCIAS DA SAUDEpt_BR
dc.titleIsolation And Characterization Of Papain-Like Cysteine Proteinases From Morinda Citrifolia Seed With Anti-Toxoplasmose Effectpt_BR
dc.typeTrabalho de Conclusão de Cursopt_BR
dspace.entity.typePublication

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
IsolationCharacterizationPapain-Like.pdf
Tamanho:
1.53 MB
Formato:
Adobe Portable Document Format
Descrição:
TCC
Baixar

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
license.txt
Tamanho:
1.75 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar

Coleções

TCC - Ciências Biomédicas