Influência do polimorfismo -196/-174 del do receptor toll-like 2 (tlr2) na susceptibilidade ao câncer de próstata

Abstract

The Toll-like family (TLR) represents the main group of receptors related to innate immune system that respond against antigenic factors, including to Damage-Associated Molecular Patterns (DAMPs), which are produced by cancerous cells. Considering the Prostate Cancer (PCa), the genetic regulation, the cell signaling, the secreted cytokines and the recruited immune cells related to TLR2 activation, still need to be elucidated. This work aimed to characterize the -196/-174del polymorphism of TLR2 in PCa susceptibility in Brazilian men. Through allele-specific PCR, 23 patients with PCa and 23 with Benign prostatic hyperplasia (BPH) were genotyped. Men were considered as Ins/Ins (Insertion/Insertion),when both alleles were present(288bp), Ins/Del as heterozygous and Del/Del (Deletion/Deletion) with both 266bp alleles. There were no statistically significant difference of age and PSA values between the studied groups (p= 0.12 and p= 0.17, respectively). The genotypic frequency was in Hardy-Weinberg Equilibrium (HWE) (p=0.90 for PCa and 0.43 for BPH). Furthermore, the genotypes did not differentiate PCa and BPH patients; however, there was a higher prevalence of deleterious allele in men affected by the disease. A greater number of individuals need to be evaluated, as well as analyzes of the transcripts number of TLR2 gene must be conducted to define the impact of this mutation in prostatic lesions.