Estudos sobre a intercalação dos corantes amaranto e eritrosina em DNA

Abstract

This work lias (br base the research aboul lhe intcrcalation proeess olthe dves aiiiaranth and erythrosiue with DNA. We present our results of an investigation of intcrcalation compound fomiation between calf thymus DNA and these colorants, frerpientlv used food colorants in Brazil. Compounds formation were accompanied by UV/Vis, electrophoreses in agarose gel and terbium(III) fluorescence as cliemical probe for DNA. Compared fo pine DNA, saniples in lhe presence of lhe colora»! show a increase in absorbance. Simultaneously, colorants absorbance's decreased. These results suggesl that colorants are incorporatcd into the DNA nnclcotide. A fluorcsccncc spectrum is showed and the results suggesl lhe sites occupied by aniaranth are the sanie as those occupied by terbiuin(III). Since the terbium probe fluorescence is priniarily due to the guanine bounding, we can conclude lliat aniaranth intercalate in DNA mainly occupying the guanine site Eletrophoreses attested DNA integrity abei its inlcraclion with aniaranth and erythrosiue.