Análise da absorção e espalhamento óptico em phantoms de tecido biológico utilizando a técnica de imagem no domínio de frequência espacial

Abstract

Currently, the most widely method used for tumor analysis in biological tissues is through biopsy, a technique that demands time, high cost and inconvenience to the patient. To reduce these disorders and to increase the effectiveness of the tumor examinations, the technique of Spatial Frequency Domain Image (SFDI) was proposed to obtain optical parameters of analysis. The SFDI technique is based on light reflection and absorption, and it can measure and map optical absorption (μa) and scattering coefficients (μs) in tissues and phantoms without direct contact, which are obtained by combining a spatially modulated periodic illumination with a camera-based imaging system. In this work we aim to use the SFDI technique to analyze biological parameters of absorption and scattering of three different chromophores: hemoglobin, lipid and water. Initially measurements were made using the integrate sphere technique, later the results were comparated with the data processing by the SFDI technique. Finally, the biological phantom image technique was applied based on avian meat and bovine fat. The results indicate that the technique is sensitive to variations in concentrations of chromophores, thus giving indications of a possible medical application in detection of tumors.