Publicação: In vitro assessment of antitumor and antiparasitic effects of extracts and fractions from Persea Americana and Syzygium cumini
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CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA, CNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIA::IMUNOLOGIA CELULAR
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Persea americana (Avocado) of the Lauraceae family and Syzygium cumini
(Jambolan) of the Myrtaceae family are food and medicinal plants widely used with antioxidant,
anti-inflammatory, antitumor, and antiparasitic activities. Objective: The present study
investigated in vitro the antitumor and antiparasitic effects of extracts and fractions purified
from P. americana and S. cumini. Materials and Methods: Fractions were obtained from the
crude ethanolic extract of P. americana seed (EtOHPaS), P. americana fruit peel (EtOHPaP)
and Syzygium cumini leaves (EtOHScL) using polar solvents: hexane (fractions FHPA and
EHSc), dichloromethane (fractions FDMPA and EDMSc), ethyl acetate (fraction FAePA), nbutanol (fractions FnBPA and EnBSc) and water (fractions FaqPA and EAqSc).
Immunohemolysis and MTT assays were performed against the HFF, Beas-2B and Pnt-2 nontumor cells to assess the cytotoxicity of the samples. To evaluate the antitumor activity, MTT
assays were performed using PC3 (prostate cancer cell) and A549 (lung cancer cell). Colony
assays were also performed to evaluate the inhibitory effect of the samples on colony formation.
Regarding the antiparasitic activity, plaque assays were performed to analyze the effect of the
samples on the invasion and proliferation process of Toxoplasma gondii tachyzoites. Results:
The results showed that the FAePA fraction of EtOHPaS exhibited high antitumor activity at a
concentration of 400 μg/ml, with a A549 tumor cell death percentage of 45%. The EtOHScL
and the EHSc and EDMSc fractions had higher antiproliferative effects against PC3 tumor cell.
Furthermore, EtOHPaP and its FHPA fraction; the FAePA, FDMPA, FaqPA fractions of
EtOHPaS; and EtOHScL and its EnBSc, EAqSc, EDMSc, and EHSc fractions demonstrated
antiparasitic properties indicating that these treatments effectively inhibited the proliferation
and reinvasion of T. gondii tachyzoites. Conclusions: In that sense, the fractions obtained from
EtOHPaP, EtOHPaS and EtOHScL show promising therapeutic potential. More studies are
required to investigate the mechanisms which may be responsible for the biological activities
of these plants.
Abstract
Palavras-chave
Syzygium cumini; Persea americana; tumor cells; Toxoplasma gondii.
Citação
BALDO, Luana Brasil. In vitro assessment of antitumor and antiparasitic effects of extracts and fractions from Persea Americana and Syzygium cumini. 2024. 26 f. Trabalho de Conclusão de Curso (Graduação em Biomedicina) – Universidade Federal de Uberlândia, Uberlândia, 2024.