https://repositorio.ufu.br/handle/123456789/5476 Fri, 18 Sep 2026 15:27:15 GMT 2026-09-18T15:27:15Z https://repositorio.ufu.br/handle/123456789/50271 Title: Infecção natural por Leptospira spp. em touros destinados a reprodução Abstract: The objective of this study was to investigate the prevalence of pathogenic Leptospira infection in breeding bulls used as semen donors. A total of 101 blood serum and semen samples were analyzed from clinically healthy bulls that were not vaccinated against leptospirosis and belonged to a reproduction center in the Triângulo Mineiro region of Minas Gerais, Brazil. Serological detection was performed using the microscopic agglutination test (MAT), and molecular detection was carried out by polymerase chain reaction (PCR) targeting the 16S rRNA, LipL32, and Loa22 genes, in addition to qPCR quantification of leptospiral DNA for the LipL32 gene. Semen quality was assessed using computer-assisted semen analysis (CASA), considering progressive motility, sperm concentration, and morphological defects. The results demonstrated seroreactivity in 13/101 (12.87%) of the bulls, with a predominance of reactions to Wolffi and Grippotyphosa. Molecular detection revealed a higher frequency for the LipL32 gene 47/101 (46.5%), demonstrating the presence of leptospiral DNA in semen, even in seronegative animals. Clinically healthy bulls may act as carriers of pathogenic Leptospira spp. Thu, 15 Jan 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/50271 2026-01-15T00:00:00Z https://repositorio.ufu.br/handle/123456789/50265 Title: Influência da frequência de mensuração da matéria seca no cálculo do consumo alimentar residual em bovinos nelore Abstract: Residual Feed Intake (RFI) is a tool that helps identify animals that are more efficient in using feed. The Feed Efficiency Manual governs the step-by-step guide for measuring it, and recommends analyzing the dry matter (DM) of the diet every 14 days. However, it is known that DM is variable and, therefore, analysis every two weeks may not represent the veracity of what is being provided to the animals. For this reason, the objective was to evaluate the DM measurement range of the total mixed ration (TMR) supplied to animals participating in the feed efficiency test. The data used in the research were collected during two feed efficiency tests, carried out at the Capim Branco experimental farm at the Federal University of Uberlândia (UFU), lasting 56 days each. The diet was composed of corn silage and concentrate, TMR DM was performed daily. When analyzing the two tests, it is possible to verify that the average daily DM (DDM) did not differ statistically from the other scenarios analyzed, which were the average DM on day 1 (DMD1), the average DM on the last day of the test (DMLD) , the average DM every 7 days (DM7D), the average DM every 14 days (DM14D) and the average MS at the beginning, middle and end of the race (DMFML). It is concluded that the 14-day collection interval guidance indicated by the Feed Efficiency Manual is correct, however, it is possible to choose to carry out only one collection, aiming to optimize the work and reduce costs with frequent analyses. Wed, 30 Aug 2023 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/50265 2023-08-30T00:00:00Z https://repositorio.ufu.br/handle/123456789/50231 Title: Influência da idade e do sexo no perfil bioquímico sérico de frangos de corte. Abstract: The objective of this study was to evaluate the influence of age and sex on the serum biochemical profile of broileres in an industrial production. Blood samples were collected from 100 broilers of the Ross genetic through unar vein, 50 females and 50 males, grouped according to age at zero, 7, 14, 23 and 28 days. The serum concentrations of total proteins, albumin, globulins, albumin/globulin, uric acid, urea, creatinine, cholesterol, triglycerides, HDL, LDL, VLDL, calcium, phosphorus, and Ca/P ratios were determined, magnesium, ALT, AST, GGT and ALP. The values between sex at the same age and the male and female group (mixed) at different ages were compared. In general, age influenced all parameters and sex in only eight of the twenty parameters evaluated. Fri, 31 Mar 2017 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/50231 2017-03-31T00:00:00Z https://repositorio.ufu.br/handle/123456789/50140 Title: Desenvolvimento de um método esfoliativo para diagnóstico de placa aural em equinos e investigação do potencial de carrapatos como vetores de papilomavírus Abstract: Aural plaque is a viral dermatological disease that affects the ear region of equines. It is caused by various papillomaviruses and presents characteristics of depigmentation and keratinization of the inner ear, which can present as punctiform or coalescent lesions.These lesions generate economic losses for horse breeders due to the commercial depreciation of the affected animals, limiting participation in some shows, competitions, and auctions. Currently, the diagnosis of these lesions relies primarily on the collection of a biopsy followed by polymerase chain reaction (PCR), the test with the highest diagnostic sensitivity for the virus. A biopsy involves using a 6- to 8-mm-diameter punch, from which a fragment of the affected tissue is removed for examination. Subsequently, the biopsied area requires daily wound care until completely healed. In this study, we used a 2-mm dermatological curette to curette the inner ear of clinically evaluated horses with aural plaques. Subsequent PCR analysis was performed, along with histopathological analysis and collection of ticks from the region. These were analyzed by PCR to investigate their relationship with papillomavirus transmission. One hundred and one clinically diagnosed aural plaque lesions were collected from 79 animals of different breeds, coats, ages, and husbandry systems located in the Uberlândia region of Minas Gerais. The results showed no statistically significant difference between the methods (p > 0.05). The exfoliative method presented sensitivity and specificity equivalent to biopsy for the EcPV1 (84.2%), EcPV3 (100%), and EcPV6 (71.4%) genotypes, with emphasis on EcPV3, which obtained excellent agreement. Coinfections were detected in 43% of the samples, the most common being between EcPV1 and EcPV6 (40.2%) and between EcPV1 and EcPV4 (25.3%). 100% of the ticks were Dermacentor nitens and tested positive for EcPV1. Coinfections occurred in 30% of the parasites, with a predominance of the combinations EcPV1 and EcPV6 (23.3%) and EcPV1 and EcPV4 (3.3%). These findings suggest that although the detection of viral DNA in ticks does not prove biological transmission, the results indicate a strong association between the presence of these ectoparasites and EcPV infection in horses. Wed, 28 Jan 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/50140 2026-01-28T00:00:00Z