https://repositorio.ufu.br/handle/123456789/5476 Tue, 07 Apr 2026 02:01:11 GMT 2026-04-07T02:01:11Z https://repositorio.ufu.br/handle/123456789/48518 Title: influência da condição corporal pré-parto na dinâmica do parto e intervenção manual em fêmeas suínas: avaliação por caliper Abstract: The purpose of this research was to evaluate the impact of pre-partum body condition score, measured using the Caliper, on the occurrence of dystocia and the effects of manual intervention associated with the duration of farrowing. Body condition scores were measured in sows using the Caliper, pre-partum and during the first three weeks of lactation. The variables were subjected to analysis of variance and Tukey's test, with a significance level of 5%. The results showed that touch was associated with increased farrowing duration and a higher incidence of stillbirths, even though sows in this group showed less body condition score loss during lactation, possibly due to post-partum therapeutic support. On the other hand, pre-partum body condition score did not significantly influence farrowing duration, number of live births, and stillbirths. Greater body condition score loss was observed among fat sows, suggesting greater mobilization of energy reserves throughout lactation. Therefore, the management of sows should prioritize good body condition at farrowing, reduction of unnecessary obstetric interventions, and efficient therapeutic support for recovery, prioritizing the well-being of the sow. Wed, 14 Jan 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48518 2026-01-14T00:00:00Z https://repositorio.ufu.br/handle/123456789/48349 Title: Interação de aditivos alimentares em dietas ricas em amido à base de sorgo para cordeiras Abstract: The objective of this study was to evaluate the combined effects of live yeast (Saccharomyces cerevisiae), condensed tannin, and an amylolytic enzyme on feed intake, diet digestibility, nitrogen balance, and blood parameters of ewe lambs fed high-starch sorghum-based diets. Five crossbred ewe lambs (Dorper × Santa Inês), with an average age of 5.6 ± 1.4 months and an initial body weight of 28.8 ± 3.7 kg, were housed in individual metabolic cages for a 75-day experimental period. The experimental diets were formulated with a 20:80 forage-to concentrate ratio, and animals were fed twice daily at 08:00 and 16:00 h. The treatments consisted of: i) diet without additives (control); ii) amylolytic enzyme + tannin (TE); iii) amylolytic enzyme + live yeast (EY); iv) live yeast + tannin (TY); and v) live yeast + tannin + amylolytic enzyme (TYE). During a five-day collection period, nutrient intake and digestibility, as well as protein, enzymatic, and energy-related blood metabolites, were evaluated. Data were analyzed using PROC MIXED of SAS, considering differences significant at P ≤ 0.05 and trends at P ≤ 0.10. The experiment was conducted in a 5 × 5 Latin square design. The inclusion of additives increased plasma glucose, dry matter (DM) intake, and the digestibility of DM and starch. The TE, TY, and TYE treatments showed higher crude protein (CP) intake compared to the EY treatment. The digestibility of DM, CP, and starch was reduced in the TY and TYE treatments, which also showed higher fecal output (as-fed basis) and higher fecal dry matter content. The EY treatment reduced nitrogen balance and increased serum LDL concentrations, whereas the TE treatment resulted in lower fecal nitrogen excretion and lower triglyceride concentrations. A tendency toward increased serum urea and creatinine concentrations was observed in the EY and TE treatments. The TYE treatment promoted higher serum alkaline phosphatase activity. No effects of the additives were observed on water intake, fecal starch content, urinary volume and density, fecal dry matter output, fecal score, or urinary nitrogen excretion. A linear effect was observed in the glycemic curve, with the highest value recorded at 17:00 h (71.03 mg dL⁻¹). It is concluded that different combinations of live yeast, condensed tannin, and amylolytic enzyme in high-concentrate diets for ewe lambs increase DM intake and improve DM and starch digestibility without compromising water balance or the evaluated energy, enzymatic, and protein metabolic parameters. Fri, 06 Feb 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48349 2026-02-06T00:00:00Z https://repositorio.ufu.br/handle/123456789/48348 Title: O embrião de galinha antes de experimentar dor poderia ser um modelo experimental adequado para infecção de Sporothrix brasiliensis e S. schenckii ? Abstract: Sporotrichosis is a chronic or subacute fungal infection transmitted by fungi of the genus Sporothrix, infecting humans and animals, especially domestic cats. The Gallus gallus domesticus embryo is suitable for scientific research because it offers an environment with more variables than in vitro models and more suitability to the 3Rs principles (Replacement, Reduction, Refinement ) than animal models with higher complexities, like the mammals. The objective of this work is to evaluate whether the chicken embryo can act as an alternative model to investigate infection by S. brasiliensis and S. schenckii. The first chapter presents a literature review on rising sporotrichosis in cats and humans, and the characteristics of the chicken embryo for a better understanding of its use as an experimental model. The second chapter is the scientific article. Our results demonstrate that, although Sporothrix spp. is not a natural avian pathogen, it is capable of causing lesions and an inflammatory response in chicken embryos. While no clear virulence differences were observed between strains based on macro- and microscopic lesions, the induction of IL-8 suggests that this chemokine may be a relevant inflammation marker 72 hours after inoculation and reinforces the suitability of this model in researches about the sporotrichosis pathogenesis and immune response. Fri, 19 Dec 2025 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48348 2025-12-19T00:00:00Z https://repositorio.ufu.br/handle/123456789/48346 Title: Can extracellular particles from different Bacillus spp. strains contribute to the inhibition and synergistic effect against Salmonella Heidelberg strains isolated from poultry? Abstract: The role of extracellular particles (EPs) in bacterial communication and pathogenesis has gained increasing attention, and studies related to probiotic strains remain scarce. This study evaluated the strain-dependent inhibitory and synergistic effects of EPs produced by Bacillus cereus (BC), Bacillus subtilis (BS), and Bacillus licheniformis (BL) against Salmonella enterica subsp. enterica serovar Heidelberg (SH). EPs were separated from the water-soluble, non-particulate (WS-NP) fraction of cell-free supernatants (CFS) obtained from Bacillus strains, cultured individually and in combination, using ultracentrifugation and ultrafiltration. EPs were characterized for particle concentration, size distribution, protein content, and metabolomic profiling to identify strain- and combination-specific metabolic signatures. Finally, the antimicrobial activity of EPs against SH was assessed. The EPs exhibited pronounced heterogeneity, with particle diameters ranging from 142.3 to 367.4 nm and high yields exceeding 10¹⁰ particles/mL after concentration. Transmission electron microscopy is consistent with the coexistence of vesicular and non-vesicular particle subpopulations. Functionally, dual EP combinations (BL+BS and BL+BC) showed synergistic effects against SH strain 13, achieving up to 99% growth inhibition. All EPs treatments reduced SH strain 18 by approximately 2 log CFU/mL, while inhibition of SH strain 06 was more limited (~1 log CFU/mL). Metabolomic profiling identified 61 metabolites, 23 of which showed differential abundance across groups, particularly those with antimicrobial and quorum-sensing activity, providing insight into the metabolic basis underlying EP-mediated interactions. Overall, these findings demonstrate strain-dependent EP-mediated inhibition of SH and their synergistic effects, highlighting the critical role of probiotic bacteria's EPs in inhibition and the contribution of their active components to this process. Mon, 26 Jan 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48346 2026-01-26T00:00:00Z