https://repositorio.ufu.br/handle/123456789/5167 Alterada nomencatura do antigo Instituto de Genética e Bioquímica: Resolução 05/99 do Conselho Universitário Sun, 05 Apr 2026 21:39:15 GMT 2026-04-05T21:39:15Z https://repositorio.ufu.br:443/retrieve/70b47b51-150e-460e-8b69-6e0d41786847/logo_ibec_vertical.png https://repositorio.ufu.br/handle/123456789/5167 https://repositorio.ufu.br/handle/123456789/48567 Title: Biorremediação utilizando microrganismos, como alternativa para o tratamento de efluentes contaminados com corantes: uma revisão. Abstract: The textile industry occupies a prominent position in the global economy. However, the fabric finishing process results in the generation of large volumes of effluents loaded with synthetic dyes, compromising the quality of water resources and aquatic life. This work aims to analyze the efficiency of bioremediation using microorganisms as a sustainable technological strategy for the degradation of these recalcitrant compounds. The methodology is based on an integrative literature review conducted in the Science Direct and Scielo databases, as well as in institutional repositories, prioritizing studies that address the application of microorganisms, focusing on fungi, and their enzymatic systems in wastewater. The results indicate that the use of bacteria and fungi, especially white-rot fungi, promotes the breakdown of the chemical structure of dyes through the action of oxidoreductase enzymes, such as laccases and peroxidases, among others, such as hydrolases, lipases, and proteases. It is identified that the stability of these biological agents is directly influenced by environmental and physicochemical variables. It is concluded that the decontamination of textile effluents containing synthetic dyes constitutes a huge challenge for the industry, especially the Textile Industry, with bioremediation promoted by microorganisms, particularly fungi that produce oxidoreductase enzymes, representing a viable alternative capable of ensuring greater sustainability throughout the production chain. The implementation of enzymatic immobilization techniques and the use of microbial consortia will additionally bring necessary advances to ensure operational robustness and enable the practical application of this system in environmental decontamination. Thu, 12 Mar 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48567 2026-03-12T00:00:00Z https://repositorio.ufu.br/handle/123456789/48561 Title: Avaliação in vitro do efeito dos pesticidas dimetomorfe e fenpropimorfe em células hepáticas humanas Abstract: Brazil is one of the largest consumers of pesticides worldwide, and some of these compounds have had their use restricted by international regulatory agences due to the lack of knowledge regarding their impacts on human health. Among the most widely used classes, fungicides receive considerable attention given their high toxicological potential. Therefore, the present study aims to evaluate, in silico and in vitro, the effects of the morpholine fungicides dimethomorph (DIM) and fenpropimorph (FEN) on two human hepatic cell lines. The ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) predictions indicated a high hepatotoxic potential and a tendency toward bioaccumulation for both compounds. Cell viability was assessed using Sulforhodamine B assay after incubation of the cells at concentrations of 300, 150, 75, 37.5, 18.75, and 9.375 µg/mL for DIM and 2, 1, 0.5, 0.25, 0.125, and 0.0625 µg/mL for FEN over a 24-hour period. For the LX-2 cell line, only the concentrations of 300 µg/mL (DIM) and 1 µg/mL (FEN) showed a significant decrease in viability. In HepG2 cells, treatments below 75 µg/mL (DIM) and above 0.25 µg/mL (FEN) resulted in reduced viability and increased production of reactive oxygen species (ROS), highlighting the possible role of such molecules in hepatotoxicity mechanisms and cell death. Additional studies are required to elucidate the molecular mechanisms involved. Fri, 06 Mar 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48561 2026-03-06T00:00:00Z https://repositorio.ufu.br/handle/123456789/48550 Title: Crescimento e descrição morfológicas das Acidobactérias AB46 e AB29 Abstract: The Cerrado is Brazil’s second-largest biome, boasting a rich microbial biodiversity and exhibiting diverse survival mechanisms. Among these microorganisms are acidobacteria, which are capable of producing compounds of industrial interest. Despite this, they are difficult to culture, with only a small number of species that can be cultured and have been taxonomically described. The objective of this study was to evaluate the growth and morphology of acidobacteria AB46 and AB29 on different carbon sources and to compare them with other previously characterized acidobacteria obtained from Cerrado soil. To this end, the morphologies of the cells and colonies were described, and growth was monitored by the formation of colony-forming units over a 20-day period in VL-55 medium supplemented with one of the following carbon sources: xylan, xylose, cellobiose, and lactose. The results demonstrated that AB29 is a coccobacillus-shaped bacterium that forms white, mucous colonies. Growth of this strain was observed in all media containing different carbon sources, with celobiose being particularly prominent during the first six days. Strain AB46 exhibited typical Gram-positive staining, a result not consistent with all other members of the phylum Acidobacteriota, furthermore, the pink staining typical of carotenoid-producing acidobacteria was not observed on all plates, indicating culture contamination. Thus, the AB29 strains appear to be distinct from the other previously characterized acidobacteria strains from Cerrado soil, and the results for the AB46 strain were incomplete due to contamination. Fri, 13 Mar 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48550 2026-03-13T00:00:00Z https://repositorio.ufu.br/handle/123456789/48506 Title: Avaliação dos efeitos de dendropsofina 1, um peptídeo natural antimicrobiano, e de dois peptídeos análogos sobre a inflamação crônica induzida por implante de esponja sintética em camundongos Abstract: Inflammation is a biological process aimed at restoring homeostasis after harmful stimuli. Through these events, the immune system removes injury-causing agents and promotes tissue repair. Although inflammatory events are essential for life, failure in their resolution may result in the development of chronic-degenerative diseases. Chronic inflammation is present in many disorders, such as diabetes mellitus, rheumatoid arthritis, cancer, and hard-to-heal wounds. In fact, many of the leading causes of death and major burdens on healthcare systems are diseases in which a persistent inflammatory state is present. Under these conditions, there is exacerbated activity of one or more components of chronic inflammation, namely inflammatory mediators, fibrogenesis, and/or angiogenesis. Continuous tissue damage and unsuccessful attempts at repair coexist in chronic inflammation. Thus, cytokines produced at the inflammatory site influence angiogenesis and extracellular matrix (ECM) deposition. Consequently, fibrosis may develop and local tissue function may be lost. Given this scenario, there is a clear need to search for biologically active compounds capable of keeping inflammation under control without completely silencing it; compounds that enable the return to homeostasis while minimizing functional loss. Antimicrobial peptides (AMPs) are a diverse group of bioactive proteins that constitute the first line of defense of various organisms against pathogens. Amphibian skin, for example, is an important reservoir of AMPs. In addition to exerting direct antimicrobial activity, these peptides may inhibit the expression of pro-inflammatory cytokines and chemokines, act as chemotactic molecules, activate immune cells, and modulate fibrogenic and vascular components of inflammation. The present study highlights the activity of a peptide isolated from the skin secretion of Dendropsophus columbianus, a tree frog endemic to Colombia, and two of its synthetic analogs. In 2018, the native peptide, named dendropsophin 1 (Dc1), was isolated, and subsequently the analogs Dc1.2 and Dc1.2.2 were synthesized. These peptides have documented antimicrobial activity; however, since their in vivo immunomodulatory action had not yet been elucidated, we evaluated the effects of their daily administration in a mouse model of chronic inflammation induced by polyester-polyurethane sponge implants. The sponge matrices induce a chronic foreign body-type inflammatory response and form fibrovascular tissue. Therefore, this model makes it possible to simultaneously evaluate inflammatory markers, angiogenesis, and fibrogenesis in vivo. To date, this is the first study to analyze the effects of dendropsophin 1 and its analogs in this context, and it demonstrates the therapeutic potential of this peptide for the treatment of conditions that require tissue repair and inflammatory control. Fri, 27 Feb 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48506 2026-02-27T00:00:00Z