https://repositorio.ufu.br/handle/123456789/21238 Wed, 22 Apr 2026 06:17:09 GMT 2026-04-22T06:17:09Z https://repositorio.ufu.br/handle/123456789/48567 Title: Biorremediação utilizando microrganismos, como alternativa para o tratamento de efluentes contaminados com corantes: uma revisão. Abstract: The textile industry occupies a prominent position in the global economy. However, the fabric finishing process results in the generation of large volumes of effluents loaded with synthetic dyes, compromising the quality of water resources and aquatic life. This work aims to analyze the efficiency of bioremediation using microorganisms as a sustainable technological strategy for the degradation of these recalcitrant compounds. The methodology is based on an integrative literature review conducted in the Science Direct and Scielo databases, as well as in institutional repositories, prioritizing studies that address the application of microorganisms, focusing on fungi, and their enzymatic systems in wastewater. The results indicate that the use of bacteria and fungi, especially white-rot fungi, promotes the breakdown of the chemical structure of dyes through the action of oxidoreductase enzymes, such as laccases and peroxidases, among others, such as hydrolases, lipases, and proteases. It is identified that the stability of these biological agents is directly influenced by environmental and physicochemical variables. It is concluded that the decontamination of textile effluents containing synthetic dyes constitutes a huge challenge for the industry, especially the Textile Industry, with bioremediation promoted by microorganisms, particularly fungi that produce oxidoreductase enzymes, representing a viable alternative capable of ensuring greater sustainability throughout the production chain. The implementation of enzymatic immobilization techniques and the use of microbial consortia will additionally bring necessary advances to ensure operational robustness and enable the practical application of this system in environmental decontamination. Thu, 12 Mar 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48567 2026-03-12T00:00:00Z https://repositorio.ufu.br/handle/123456789/48561 Title: Avaliação in vitro do efeito dos pesticidas dimetomorfe e fenpropimorfe em células hepáticas humanas Abstract: Brazil is one of the largest consumers of pesticides worldwide, and some of these compounds have had their use restricted by international regulatory agences due to the lack of knowledge regarding their impacts on human health. Among the most widely used classes, fungicides receive considerable attention given their high toxicological potential. Therefore, the present study aims to evaluate, in silico and in vitro, the effects of the morpholine fungicides dimethomorph (DIM) and fenpropimorph (FEN) on two human hepatic cell lines. The ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) predictions indicated a high hepatotoxic potential and a tendency toward bioaccumulation for both compounds. Cell viability was assessed using Sulforhodamine B assay after incubation of the cells at concentrations of 300, 150, 75, 37.5, 18.75, and 9.375 µg/mL for DIM and 2, 1, 0.5, 0.25, 0.125, and 0.0625 µg/mL for FEN over a 24-hour period. For the LX-2 cell line, only the concentrations of 300 µg/mL (DIM) and 1 µg/mL (FEN) showed a significant decrease in viability. In HepG2 cells, treatments below 75 µg/mL (DIM) and above 0.25 µg/mL (FEN) resulted in reduced viability and increased production of reactive oxygen species (ROS), highlighting the possible role of such molecules in hepatotoxicity mechanisms and cell death. Additional studies are required to elucidate the molecular mechanisms involved. Fri, 06 Mar 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48561 2026-03-06T00:00:00Z https://repositorio.ufu.br/handle/123456789/48550 Title: Crescimento e descrição morfológicas das Acidobactérias AB46 e AB29 Abstract: The Cerrado is Brazil’s second-largest biome, boasting a rich microbial biodiversity and exhibiting diverse survival mechanisms. Among these microorganisms are acidobacteria, which are capable of producing compounds of industrial interest. Despite this, they are difficult to culture, with only a small number of species that can be cultured and have been taxonomically described. The objective of this study was to evaluate the growth and morphology of acidobacteria AB46 and AB29 on different carbon sources and to compare them with other previously characterized acidobacteria obtained from Cerrado soil. To this end, the morphologies of the cells and colonies were described, and growth was monitored by the formation of colony-forming units over a 20-day period in VL-55 medium supplemented with one of the following carbon sources: xylan, xylose, cellobiose, and lactose. The results demonstrated that AB29 is a coccobacillus-shaped bacterium that forms white, mucous colonies. Growth of this strain was observed in all media containing different carbon sources, with celobiose being particularly prominent during the first six days. Strain AB46 exhibited typical Gram-positive staining, a result not consistent with all other members of the phylum Acidobacteriota, furthermore, the pink staining typical of carotenoid-producing acidobacteria was not observed on all plates, indicating culture contamination. Thus, the AB29 strains appear to be distinct from the other previously characterized acidobacteria strains from Cerrado soil, and the results for the AB46 strain were incomplete due to contamination. Fri, 13 Mar 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48550 2026-03-13T00:00:00Z https://repositorio.ufu.br/handle/123456789/48479 Title: Detecção molecular de espécie de fungo em amostras de sangue de pacientes com câncer de próstata Abstract: Prostate cancer (PCa) is the most common malignant neoplasm among men worldwide and, in most cases, is diagnosed at an advanced stage, especially in countries with a low Human Development Index. Consequently, the prognosis of the disease is unfavorable, with significant mortality rates, emphasizing the need for new screening and diagnostic approaches. Recent studies have demonstrated the role of the microbiome in the onset and progression of PCa; however, fungal species have not yet been correlated with the disease. In this study, we aimed to detect and evaluate the clinical potential of fungal species in blood samples from patients with PCa and benign prostatic hyperplasia (BPH). To this end, analyses were conducted using databases from Harvard University, Washington, MIT, and North Carolina to select species associated with PCa. Primers were designed for Malassezia globosa, and qPCR reactions were performed on blood samples from patients with PCa (n = 40) and BPH (n = 32). M. globosa did not differentiate patients with PCa from those with BPH, and no statistically significant correlations were observed with age, preoperative PSA of 4 ng/mL, Gleason score, or TNM staging. However, the presence of the fungus was statistically higher in patients with preoperative PSA > 10 ng/mL (p = 0.0108). These findings are pioneering in demonstrating an association between M. globosa and elevated preoperative PSA levels in PCa. Thus, studies focused on characterizing the prostatic mycobiome may open new pathways for understanding the disease and for the development of clinically applicable strategies. Tue, 03 Mar 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48479 2026-03-03T00:00:00Z