https://repositorio.ufu.br/handle/123456789/21238 Mon, 20 Jul 2026 04:06:17 GMT 2026-07-20T04:06:17Z https://repositorio.ufu.br/handle/123456789/48899 Title: Avaliação do efeito de diferentes fontes de carbono na produção de xilanase por acidobactérias Tue, 10 Mar 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48899 2026-03-10T00:00:00Z https://repositorio.ufu.br/handle/123456789/48801 Title: Avaliação dos efeitos dos fungicidas fenpropimorfe e dimetomorfe em células tumorais mamárias e prostáticas Abstract: Breast cancer and prostate cancer represent major public health challenges in Brazil, with estimated incidences of 78,610 and 77,920 new cases, respectively, for the 2026–2028 triennium. These neoplasms are associated with multiple risk factors, including physical, biological, and chemical agents. Among these, agrochemicals stand out, as they are widely used in agriculture for pest control and to increase productivity. Despite their relevance in the agricultural sector, the potential impacts of these compounds on human health remain incompletely understood, particularly regarding fungicides. In this context, the present study aimed to evaluate the functional and molecular effects of the fungicides Fenpropimorph and Dimethomorph in tumor and non-tumor breast cell lines (MCF-7, MDA-MB-231, and MCF10A) and prostate cell lines (LNCaP, PC-3, and PNT-2), as well as in human fibroblasts (HFF). After the initial viability assay using MTT, the concentrations of 0.125 µM and 0.250 µM of Fenpropimorph were selected for subsequent analyses in the MCF-7 luminal breast cancer cell line after 48 hours of exposure. Dimethomorph showed cytotoxic effect, reducing MTT metabolism at both evaluated time points. The lactate dehydrogenase release assay indicated that Fenpropimorph did not promote significant cytotoxicity at the tested concentrations. However, an increase in Caspase-8 activity, determined by enzymatic assay, was observed, suggesting possible activation of apoptosis-related pathways. In the clonogenic assay, a reduction in the proliferative potential of MCF-7 cells was observed, and treatment with 0.250 µM for 48 hours resulted in increased horizontal migration in the wound healing assay. Finally, quantitative real-time polymerase chain reaction analysis revealed increased expression of Interleukin-1 beta when cells were treated with 0.125 µM of Fenpropimorph. These findings suggest that Fenpropimorph may modulate inflammatory pathways and processes associated with tumor progression in MCF-7 cells. However, additional studies are necessary to further elucidate the molecular mechanisms involved, particularly those related to hormonal signaling pathways Fri, 06 Mar 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48801 2026-03-06T00:00:00Z https://repositorio.ufu.br/handle/123456789/48567 Title: Biorremediação utilizando microrganismos, como alternativa para o tratamento de efluentes contaminados com corantes: uma revisão. Abstract: The textile industry occupies a prominent position in the global economy. However, the fabric finishing process results in the generation of large volumes of effluents loaded with synthetic dyes, compromising the quality of water resources and aquatic life. This work aims to analyze the efficiency of bioremediation using microorganisms as a sustainable technological strategy for the degradation of these recalcitrant compounds. The methodology is based on an integrative literature review conducted in the Science Direct and Scielo databases, as well as in institutional repositories, prioritizing studies that address the application of microorganisms, focusing on fungi, and their enzymatic systems in wastewater. The results indicate that the use of bacteria and fungi, especially white-rot fungi, promotes the breakdown of the chemical structure of dyes through the action of oxidoreductase enzymes, such as laccases and peroxidases, among others, such as hydrolases, lipases, and proteases. It is identified that the stability of these biological agents is directly influenced by environmental and physicochemical variables. It is concluded that the decontamination of textile effluents containing synthetic dyes constitutes a huge challenge for the industry, especially the Textile Industry, with bioremediation promoted by microorganisms, particularly fungi that produce oxidoreductase enzymes, representing a viable alternative capable of ensuring greater sustainability throughout the production chain. The implementation of enzymatic immobilization techniques and the use of microbial consortia will additionally bring necessary advances to ensure operational robustness and enable the practical application of this system in environmental decontamination. Thu, 12 Mar 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48567 2026-03-12T00:00:00Z https://repositorio.ufu.br/handle/123456789/48561 Title: Avaliação in vitro do efeito dos pesticidas dimetomorfe e fenpropimorfe em células hepáticas humanas Abstract: Brazil is one of the largest consumers of pesticides worldwide, and some of these compounds have had their use restricted by international regulatory agences due to the lack of knowledge regarding their impacts on human health. Among the most widely used classes, fungicides receive considerable attention given their high toxicological potential. Therefore, the present study aims to evaluate, in silico and in vitro, the effects of the morpholine fungicides dimethomorph (DIM) and fenpropimorph (FEN) on two human hepatic cell lines. The ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) predictions indicated a high hepatotoxic potential and a tendency toward bioaccumulation for both compounds. Cell viability was assessed using Sulforhodamine B assay after incubation of the cells at concentrations of 300, 150, 75, 37.5, 18.75, and 9.375 µg/mL for DIM and 2, 1, 0.5, 0.25, 0.125, and 0.0625 µg/mL for FEN over a 24-hour period. For the LX-2 cell line, only the concentrations of 300 µg/mL (DIM) and 1 µg/mL (FEN) showed a significant decrease in viability. In HepG2 cells, treatments below 75 µg/mL (DIM) and above 0.25 µg/mL (FEN) resulted in reduced viability and increased production of reactive oxygen species (ROS), highlighting the possible role of such molecules in hepatotoxicity mechanisms and cell death. Additional studies are required to elucidate the molecular mechanisms involved. Fri, 06 Mar 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/48561 2026-03-06T00:00:00Z