https://repositorio.ufu.br/handle/123456789/19157 Fri, 16 Oct 2026 08:59:44 GMT 2026-10-16T08:59:44Z https://repositorio.ufu.br/handle/123456789/50604 Title: Efeitos do extrato bruto e da fração acetato de etila das folhas de Eugenia dysenterica DC. na oxidação e glicação da LDL in vitro. Abstract: Atherosclerosis and diabetes mellitus are associated with the oxidation and glycation of low density lipoprotein (LDL), processes that are central to the progression of cardiometabolic diseases. Natural compounds rich in polyphenols have been investigated as potential antioxidant and antiglycation agents. Eugenia dysenterica DC., a species native to the Brazilian Cerrado, presents a high content of phenolic compounds with recognized biological activity. The present study evaluated the effect of the crude ethanolic extract and the ethyl acetate fraction from the leaves of Eugenia dysenterica DC. on the oxidation and glycation of human LDL in vitro. The samples were characterized by HPLC-ESI-MS/MS and quantified for total phenols, flavonoids, and condensed tannins. Antioxidant activity was evaluated by DPPH, ORAC, FRAP, and oxidative hemolysis. LDL oxidation was induced by Cu²⁺ and monitored by the formation of conjugated dienes and TBARS, while glycation was induced by methylglyoxal, with evaluation of AGE formation by fluorescence. The ethyl acetate fraction presented a higher content of phenolic compounds (469.5 ± 20.0 µg/mL) compared to the crude extract (218.3 ± 29.1 µg/mL), as well as higher antioxidant activity in the DPPH assay (IC₅₀ = 5.94 ± 1.63 µg/mL). In the LDL assays, both extracts significantly reduced Cu²⁺-induced oxidation, with an increase in oxidative lag time (Tlag), with the effect being more pronounced for the ethyl acetate fraction. In addition, concentration-dependent inhibition of AGE formation was observed, reaching 92.18 ± 0.79% inhibition for the ethyl acetate fraction at the highest tested concentration. The results indicate that the ethyl acetate fraction from the leaves of Eugenia dysenterica DC. presents relevant antioxidant and antiglycation activity in an in vitro experimental model, suggesting potential for future investigations in more complex biological systems related to atherosclerosis. Tue, 10 Mar 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/50604 2026-03-10T00:00:00Z https://repositorio.ufu.br/handle/123456789/50489 Title: Análise computacional da expressão de correceptores de SARS-CoV-2 em células estromais do microambiente do melanoma Murino B16F10 Abstract: The COVID-19 pandemic highlighted the need to better understand the mechanisms of viral entry into host cells, particularly given the impact of SARS-CoV-2 infection on cancer patients. Although viral entry is primarily associated with the interaction between angiotensin-converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2), studies have demonstrated that cellular coreceptors, molecules that facilitate or modulate viral adhesion, internalization, and dissemination, also play important roles in this process. In the context of cancer, these molecules are involved in biological processes such as cell adhesion, angiogenesis, extracellular matrix remodeling, and tumor progression, particularly within the tumor microenvironment. Therefore, the present study aimed to computationally analyze the expression of SARS-CoV-2 receptors and coreceptors in stromal cells of the murine B16F10 melanoma microenvironment using publicly available single-cell RNA sequencing (scRNA-seq) data. The genes Eca2, Tmprss2, Itgb1, Itgb3, Dpp4, Axl, Hspa5, Bsg, Nrp1, Vim, Hspg2, and Tlr4 were evaluated and normalized to the Actb gene. Data were obtained from the Murine Melanoma Atlas platform and analyzed using FIJI/ImageJ software through custom-developed macros to quantify relative gene expression in expression maps by applying image processing techniques, including grayscale conversion, intensity thresholding, and quantification of the labeled area. In addition to the overall analysis of the tumor microenvironment, a segmented analysis was performed in which cancer-associated fibroblasts (CAFs) and tumor endothelial cells were isolated and analyzed separately, allowing comparison of expression profiles between these stromal cell populations. The results demonstrated low expression of Eca2 and Tmprss2 within the tumor microenvironment, whereas the coreceptors exhibited heterogeneous and cell type-dependent expression patterns. Genes such as Hspa5, Bsg, Vim, and Itgb1 showed widespread expression, whereas Axl, Dpp4, Itgb3, and Tlr4 displayed more restricted expression profiles. In the segmented analysis, Bsg, Vim, Hspa5, Itgb1, and Axl were more highly expressed in CAFs, whereas Vim, Bsg, Itgb1, Hspa5, and Nrp1 were more highly expressed in tumor endothelial cells. Additionally, comparative analysis of neuropilin-1 revealed a high degree of conservation across different species. These findings indicate that SARS-CoV-2 coreceptors exhibit distinct expression patterns within the tumor microenvironment and are associated with biological processes related to stromal activation, angiogenesis, cell–matrix interactions, and tumor progression. The computational characterization of these molecules expands the understanding of their distribution within the melanoma microenvironment and provides a foundation for future experimental investigations into their role in tumor biology and their potential as therapeutic targets. Wed, 27 May 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/50489 2026-05-27T00:00:00Z https://repositorio.ufu.br/handle/123456789/50241 Title: Análise morfológica do córtex motor e do giro denteado em animais hiperlipidêmicos e tratados com Maytenus ilicifolia Abstract: Hyperlipidemia is a metabolic disorder characterized by elevated circulating lipid levels and is associated with systemic inflammation, oxidative stress, and the development of structural alterations in the central nervous system. In this context, natural compounds with antioxidant and anti-inflammatory properties, such as Maytenus ilicifolia (espinheira-santa), have attracted interest due to their potential neuroprotective effects. The present study aimed to investigate morphometric differences in the motor cortex and dentate gyrus of the hippocampus in normolipidemic, hyperlipidemic, and hyperlipidemic mice treated with an oral gavage of the ethanolic extract of Maytenus ilicifolia to verify if there would be differences in these regions that could be related to neural diseases. Sixteen C57BL/6 mice were divided into four experimental groups: normolipidemic, hyperlipidemic, hyperlipidemic treated with 100 and 400 mg/kg of the extract. Hyperlipidemia was induced with Tyloxapol for 21 days. After euthanasia, the brains were collected and processed for histological analysis using Nissl staining. The morphological analyses included measurements of motor cortex thickness, dentate gyrus layer thickness, neuronal area and density, performed using ImageJ software. The results demonstrated a significant reduction in cortical thickness in hyperlipidemic animals compared with normolipidemic animals, without changes in cortical neuronal density. In the dentate gyrus, animals treated with 100 mg/kg of Maytenus ilicifolia showed greater thickness of the granular layer, as well as a tendency toward preservation of the molecular layer compared with untreated hyperlipidemic animals. These findings suggest that hyperlipidemia promotes morphometric alterations in the brain and that Maytenus ilicifolia exhibits potential neuroprotective effects. Mon, 27 Jul 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/50241 2026-07-27T00:00:00Z https://repositorio.ufu.br/handle/123456789/50061 Title: Harmonização da imagem facial: uma análise crítica da proporção áurea no visagismo e na maquiagem Abstract: The pursuit of facial harmony has been studied across different fields of knowledge, involving mathematical, aesthetic and artistic concepts. Among the models used to interpret facial harmony, the golden ratio stands out as a mathematical relationship historically associated with the perception of balance and beauty and later incorporated into the analysis of human body proportions. However, its validity as a universal parameter of facial attractiveness remains controversial. In the field of facial aesthetics, the analysis of facial proportions allows an understanding of the relationships between facial structures and their influence on the perception of harmony and symmetry. In parallel, visagism emerges as an approach aimed at constructing personal image based on the analysis of the individual's physical characteristics and identity. In this context, make-up may be used as a tool capable of visually modifying facial proportions through techniques of light, shadow and shape correction. This study aimed to critically analyse the validity of the golden ratio as a parameter of facial harmony, considering its relationship with anthropometry, beauty perception, visagism and the use of make-up as a resource for visual modulation of the facial image. An integrative literature review with a qualitative and descriptive approach was carried out, based on studies addressing the relationship between facial proportions, image construction and make-up techniques applied to facial aesthetics. The evidence analysed indicates that, although anthropometric and morphometric parameters are useful for the objective assessment of the face, the golden ratio lacks consistent scientific support as a universal standard of beauty. The perception of facial harmony is multifactorial and influenced by morphological, population, cultural and individual characteristics. Visagism and make-up may therefore contribute to the harmonisation of the facial image when applied individually, valuing each person's own features rather than rigidly applying universal mathematical models. Wed, 05 Aug 2026 00:00:00 GMT https://repositorio.ufu.br/handle/123456789/50061 2026-08-05T00:00:00Z