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Lipoxina A4 e Resolvina D1 preservam a capacidade de indução neural de células-tronco da polpa dentária cultivadas sob condições inflamatórias

2026-02-23T00:00:00Z

Title: Lipoxina A4 e Resolvina D1 preservam a capacidade de indução neural de células-tronco da polpa dentária cultivadas sob condições inflamatórias Abstract: Human dental pulp stem cells (DPSCs) exhibit neurogenic differentiation potential; however, inflammatory conditions may impair this process. This study investigated the impact of tumor necrosis factor-alpha (TNF-α) on DPSC neurodifferentiation and the modulatory role of the pro-resolving lipid mediators lipoxin A4 (LXA4) and resolvin D1 (RvD1). DPSCs were isolated and subjected to neuronal differentiation for 21 days. The optimal TNF-α concentration was determined in a pilot study using real-time PCR. The expression of OCT3/4, doublecortin, and β-III-tubulin was analyzed by MTT assay, flow cytometry, and immunofluorescence. The effects of LXA4 (10 or 100 nM) and RvD1 (10 or 100 nM) were assessed by flow cytometry. Data were analyzed using the Kruskal Wallis test followed by the Mann–Whitney test (p<0.05) and one-way ANOVA with Tukey’s post hoc test. TNF-α at a concentration of 25 ng/mL was shown to induce inflammation without causing cytotoxicity. The high production of doublecortin indicated the neuronal differentiation potential of DPSCs, whereas reduced β-III-tubulin expression demonstrated that TNF-α inhibits the progression of neuronal differentiation. Conversely, increased β-III-tubulin expression in the presence of LXA4 (10 and 100 nM; p=0.02 and p=0.03, respectively) and RvD1 (100 nM; p=0.02) indicate that these mediators attenuate the inhibitory effects of TNF-α. These findings suggest that anti-inflammatory therapy combined with DPSCs may enhance motor recovery following neurotrauma.

Manifestações orais de doenças imunomediadas: evidências clínicas e epidemiológicas de um serviço de referência regional

2026-08-26T00:00:00Z

Title: Manifestações orais de doenças imunomediadas: evidências clínicas e epidemiológicas de um serviço de referência regional Abstract: This study addresses immune-mediated diseases with oral manifestations, a heterogeneous group of conditions that significantly impact patients' quality of life and pose diagnostic challenges due to overlapping clinical features among different diseases. The research was conducted at a regional referral center, the Dental Hospital of the Federal University of Uberlândia, and comprised two complementary investigations. The first consisted of a retrospective observational analysis of the clinical, demographic, and therapeutic characteristics of patients diagnosed with oral lichen planus. The second explored the coexistence of psoriasis and oral pemphigus vulgaris, discussing potential underlying immunopathological mechanisms. Together, these studies highlight the importance of clinicopathological correlation, multidisciplinary management, and longitudinal follow-up in the care of patients with oral immune-mediated diseases, contributing to the improvement of diagnostic and therapeutic strategies within the Brazilian Unified Health System.

Terapia a laser de baixa intensidade e gel à base de fluoreto, sílica e cálcio para o controle da hipersensibilidade dentinária: estudo clínico randomizado controlado.

2026-07-27T00:00:00Z

Title: Terapia a laser de baixa intensidade e gel à base de fluoreto, sílica e cálcio para o controle da hipersensibilidade dentinária: estudo clínico randomizado controlado. Abstract: The present study aimed to evaluate the isolated and combined effects of a fluoride/silica/calcium (FSC) gel and low-level laser therapy (LLLT) in the management of dentin hypersensitivity (DH). Forty-five patients with DH presenting at least one tooth with exposed cervical dentin on the buccal surface were included. DH was confirmed by a positive response of ≥40 mm on the Visual Analog Scale (VAS) following air and water stimulation. Participants were randomly allocated to three groups: FSC (fluoride/silica/calcium gel), LLLT (low-level laser therapy), and FSC/LLLT (combined therapy). The FSC and FSC/LLLT groups received three in-office applications of the FSC gel at 72-hour intervals, combined with home use of the gel for two months, followed by replacement with a fluoride/silica gel (without the calcium booster) for three months. The LLLT and FSC/LLLT groups received laser applications during the first three treatment sessions and at follow-up visits after 7 days, 15 days, 1 month, and 3 months. Pain was assessed following evaporative and tactile stimuli using the VAS and the Schiff Cold Air Sensitivity Scale at baseline, immediately after each treatment session, and at the 7-day, 15-day, 1-month, and 3-month follow-up visits. Oral health-related quality of life was assessed using the Dentine Hypersensitivity Experience Questionnaire (DHEQ-15) at baseline and throughout the follow-up period. All groups exhibited an immediate reduction in pain elicited by evaporative stimulation after the first intervention, with no significant differences among treatments. A similar pattern was observed after the second and third treatment sessions. Compared with baseline, all groups showed a gradual reduction in pain over time. For tactile sensitivity and Schiff scale assessments, only the LLLT group demonstrated significant reductions immediately after the first three treatment sessions, whereas all groups exhibited significant improvements over time. Oral health-related quality of life also improved in all groups. It can be concluded that both the FSC gel and LLLT were effective in reducing pain and improving the quality of life of patients with DH. However, the combination of these therapies did not provide additional benefits compared with either treatment alone, suggesting similar clinical efficacy among the treatment approaches.

Efeito da fotobiomodulação infravermelha associada à proteína da matriz do esmalte na formação óssea com osso bovino desproteinizado em ratos

2026-04-27T00:00:00Z

Title: Efeito da fotobiomodulação infravermelha associada à proteína da matriz do esmalte na formação óssea com osso bovino desproteinizado em ratos Abstract: Objective: This study evaluated whether the combination of infrared photobiomodulation therapy (IR-PBMT) and enamel matrix derivative (EMD) enhances the repair of areas grafted with deproteinized bovine bone (DBB). Material and methods: Forty-eight rats were used. Surgery was performed bilaterally in the mandible, with both sides allocated to the same experimental group. The animals were evaluated at two experimental periods (30 and 90 days; n = 6). A dome shaped Teflon was placed in the mandibular ramus of each animal, bilaterally, and filled with DBB. The groups were divided according to the type of treatment applied to the grafted area: DBB: no adjunct treatment; EMD: DBB associated with EMD; IR-PBMT: DBB subjected to IR-PBMT; IRPBMT/EMD: DBB associated with EMD + IR-PBMT. The following analyses were performed: (1) micro-computed tomography, to evaluate the volume and microstructure of the grafted area, and (2) histomorphometry, to assess the composition of the repaired tissue. Results: The DBB and IR-PBMT/EMD groups showed greater mineralized tissue volume and smaller trabecular spacing compared to the other groups (p<0.05). The DBB group showed a smaller amount of bone than all other groups at 30 days (p<0.05). The IR-PBMT/EMD group showed a greater amount of bone than the other groups at 90 days (p<0.05). Conclusion: The combined treatment with IRPBMT and EMD enhances repair in areas grafted with DBB.