https://repositorio.ufu.br/handle/123456789/5476 2026-10-09T03:13:29Z https://repositorio.ufu.br/handle/123456789/50355 Title: Padronização da técnica de biópsia renal em felinos: rendimento glomerular de acordo com calibre da agulha, trajetória e região de coleta Abstract: Renal biopsy is an effective procedure for diagnosing kidney diseases, guiding therapeutic decisions, and establishing prognosis; however, a minimum of 5–20 glomeruli is required for diagnostic adequacy. This study aimed to standardize the feline renal biopsy technique by identifying the ideal needle gauge and trajectory, as well as the renal region that maximizes glomerular yield while minimizing complications. Five feline cadavers without renal abnormalities were used. Four animals underwent renal biopsy using Tru-Cut needles (14G, 16G, and 18G) at the cranial pole, caudal pole, and lateral margin, following caudocranial and lateromedial trajectories (Tru-Cut group). One animal had its kidneys sectioned for virtual analysis, which involved glomerular counting using ImageJ software (virtual biopsy group), simulating biopsy samples obtained with specific needle gauges and renal regions. Statistical analysis included Friedman and paired Wilcoxon tests to compare needle gauges, intraclass correlation coefficient (ICC) to assess reproducibility, and logistic regression with generalized estimating equations (GEE) to evaluate complications such as needle transfixation and sample fragmentation. The results demonstrated a consistent hierarchy of glomerular yield: 14G > 16G > 18G, with statistically significant differences among all gauges. The caudocranial trajectory yielded a higher number of glomeruli than the lateromedial trajectory. Analysis by renal region showed that the lateral region consistently presented higher mean glomerular counts for the 16G and 18G needles. The ICC indicated moderate to good reproducibility for the 16G needle, whereas the 18G needle showed greater variability. The 16G needle was associated with a lower risk of sample fragmentation compared to the 18G, while the 14G needle caused macroscopic renal lesions in all punctures. The virtual biopsy model reproduced the hierarchical pattern among needle gauges but overestimated glomerular yield, indicating that technical factors reduce sampling efficiency. It was concluded that, in felines, renal biopsy should be performed in the lateral renal region, using a caudocranial trajectory and a 16G needle, in order to maximize glomerular yield and minimize the risk of complications. 2026-01-08T00:00:00Z https://repositorio.ufu.br/handle/123456789/50271 Title: Infecção natural por Leptospira spp. em touros destinados a reprodução Abstract: The objective of this study was to investigate the prevalence of pathogenic Leptospira infection in breeding bulls used as semen donors. A total of 101 blood serum and semen samples were analyzed from clinically healthy bulls that were not vaccinated against leptospirosis and belonged to a reproduction center in the Triângulo Mineiro region of Minas Gerais, Brazil. Serological detection was performed using the microscopic agglutination test (MAT), and molecular detection was carried out by polymerase chain reaction (PCR) targeting the 16S rRNA, LipL32, and Loa22 genes, in addition to qPCR quantification of leptospiral DNA for the LipL32 gene. Semen quality was assessed using computer-assisted semen analysis (CASA), considering progressive motility, sperm concentration, and morphological defects. The results demonstrated seroreactivity in 13/101 (12.87%) of the bulls, with a predominance of reactions to Wolffi and Grippotyphosa. Molecular detection revealed a higher frequency for the LipL32 gene 47/101 (46.5%), demonstrating the presence of leptospiral DNA in semen, even in seronegative animals. Clinically healthy bulls may act as carriers of pathogenic Leptospira spp. 2026-01-15T00:00:00Z https://repositorio.ufu.br/handle/123456789/50265 Title: Influência da frequência de mensuração da matéria seca no cálculo do consumo alimentar residual em bovinos nelore Abstract: Residual Feed Intake (RFI) is a tool that helps identify animals that are more efficient in using feed. The Feed Efficiency Manual governs the step-by-step guide for measuring it, and recommends analyzing the dry matter (DM) of the diet every 14 days. However, it is known that DM is variable and, therefore, analysis every two weeks may not represent the veracity of what is being provided to the animals. For this reason, the objective was to evaluate the DM measurement range of the total mixed ration (TMR) supplied to animals participating in the feed efficiency test. The data used in the research were collected during two feed efficiency tests, carried out at the Capim Branco experimental farm at the Federal University of Uberlândia (UFU), lasting 56 days each. The diet was composed of corn silage and concentrate, TMR DM was performed daily. When analyzing the two tests, it is possible to verify that the average daily DM (DDM) did not differ statistically from the other scenarios analyzed, which were the average DM on day 1 (DMD1), the average DM on the last day of the test (DMLD) , the average DM every 7 days (DM7D), the average DM every 14 days (DM14D) and the average MS at the beginning, middle and end of the race (DMFML). It is concluded that the 14-day collection interval guidance indicated by the Feed Efficiency Manual is correct, however, it is possible to choose to carry out only one collection, aiming to optimize the work and reduce costs with frequent analyses. 2023-08-30T00:00:00Z https://repositorio.ufu.br/handle/123456789/50231 Title: Influência da idade e do sexo no perfil bioquímico sérico de frangos de corte. Abstract: The objective of this study was to evaluate the influence of age and sex on the serum biochemical profile of broileres in an industrial production. Blood samples were collected from 100 broilers of the Ross genetic through unar vein, 50 females and 50 males, grouped according to age at zero, 7, 14, 23 and 28 days. The serum concentrations of total proteins, albumin, globulins, albumin/globulin, uric acid, urea, creatinine, cholesterol, triglycerides, HDL, LDL, VLDL, calcium, phosphorus, and Ca/P ratios were determined, magnesium, ALT, AST, GGT and ALP. The values between sex at the same age and the male and female group (mixed) at different ages were compared. In general, age influenced all parameters and sex in only eight of the twenty parameters evaluated. 2017-03-31T00:00:00Z