https://repositorio.ufu.br/handle/123456789/18938 2026-07-25T06:38:01Z https://repositorio.ufu.br/handle/123456789/49052 Title: Expressão aumentada da H3K14ac, HDAC2 e HDAC6 como marcador epigenético de transformação maligna de quelite actínica para carcinoma de células escamosas de lábio Abstract: Ultraviolet radiation (UVR) acts as the primary carcinogenic agent in the lower lip, triggering a cascade of genetic events and complex epigenetic modifications that culminate in malignant transformation. Among these modifications, histone acetylation and histone deacetylase (HDAC) activity emerge as critical regulators of gene expression and genomic stability. This study aimed to evaluate, through immunohistochemistry, the acetylation profile of histone H3 at lysine 14 (H3K14ac) and the expression of HDAC2 and HDAC6 enzymes in a biological and temporal series of lip carcinogenesis. The sample comprised 97 paraffin-embedded specimens, categorized into: Normal Mucosa (NM; n=15), Actinic Cheilitis (AC; n=43), and Lip Squamous Cell Carcinoma (LSCC; n=39). Quantitative and semi-quantitative analysis revealed that H3K14ac exhibits a distinct biphasic dynamic: a drastic reduction in acetylation levels was observed in AC (26.3%) compared to NM, followed by a significant increase in LSCC (48.8%; p < 0.001), suggesting specific chromatin reprogramming during neoplastic invasion. Simultaneously, HDAC2 (predominantly nuclear) and HDAC6 (cytoplasmic) enzymes demonstrated progressive, linear overexpression with increasing severity of epithelial dysplasia, reaching peak intensity in LSCC (68.9% and 82% intense scores, respectively; p < 0.001). ROC (Receiver Operating Characteristic) curve analysis confirmed the high diagnostic value of the markers, notably for HDAC6, which presented an Area Under the Curve (AUC) of 0.864, indicating excellent discriminatory capacity between pre-malignant and malignant tissues. These findings indicate that histone code dysregulation and exacerbated HDAC activity are early and persistent events in UVR-induced lip carcinogenesis, positioning these epigenetic mechanisms as potential progression biomarkers and promising therapeutic targets for the clinical management of lip neoplasms. 2026-03-26T00:00:00Z https://repositorio.ufu.br/handle/123456789/49037 Title: Perfil Metabolômico e Lipidômico do Soro de Indivíduos Com e Sem Obesidade: Contribuições para a Compreensão do Eixo Obesidade-Câncer Abstract: Obesity is a multifactorial condition associated with systemic metabolic alterations and is recognized as a risk factor for the development of different types of cancer. Considering the metabolic heterogeneity of obesity and the proposed distinction between metabolically healthy obesity and obesity associated with comorbidities, the present study aimed to evaluate and compare the metabolomic and lipidomic profiles in the serum of individuals without obesity, with obesity, and with obesity associated with comorbidities, investigating whether the observed alterations are related to metabolic pathways and molecules associated with carcinogenesis. The study population was characterized according to demographic and anthropometric variables, including age, sex, and body mass index (BMI). Metabolomic and lipidomic analyses were performed by mass spectrometry, using multivariate (PCA and PLS-DA) and univariate approaches to identify metabolites and lipids differentially expressed among the groups. Additionally, associations between the abundance of these molecules and BMI were investigated, as well as the impact of biological sex and different degrees of obesity on the analyzed profiles. The results demonstrated that individuals with obesity exhibit consistent alterations in metabolites and lipids mainly related to inflammation, oxidative stress, and lipid remodeling, including compounds positively correlated with BMI. No exclusive metabolomic or lipidomic profile was identified for obesity associated with comorbidities, suggesting that the observed alterations reflect a shared metabolic pattern throughout the progression of obesity. Taken together, these findings reinforce the relevance of lipid and oxidative metabolism in the obesity–cancer axis and contribute to a better understanding of the metabolic mechanisms associated with tumor risk in obese individuals. 2026-01-30T00:00:00Z https://repositorio.ufu.br/handle/123456789/48270 Title: Influência da proteína de choque térmico de 70 kDa de Toxoplasma gondii (TgHSP 70) na modulação da apoptose de células trofoblásticas (Linhagem BeWo) Abstract: Toxoplasmosis is an important zoonotic disease due to ability of its causal agent, Toxoplasma gondii, to infect large number of vertebrates and to be associated with congenital infection. Additionally, the parasite requires the heat shock protein of 70 kDa (TgHSP70) for stage conversion. HSP 70 protein displays the ability to modulate various signaling pathways, including the apoptosis by due anti apoptotic mechanisms. Thus, the objective of this study was to investigate the role of heat shock protein of T. gondii (TgHSP 70) on BeWo trophbastic cell line. In order to determine whether TgHSP70 can prevent apoptosis arsenic trioxide (As2O3) and arsenite sodium (NaAs02) were used to induce apoptosis on BeWo and infected with the RH and ME49 strains. To achieve the effect of TgHSP70 on apoptosis, purification and treatment of cells with recombinant TgHSP70 was carried out. Immunocytochemical assay using M30 antibody was performed to evaluated the expression of cytokeratin 18. Also As2O3 was used to induce apoptosis in BeWo cells. This cells their and followed by ME 49 and RH strains infection. The amount of TgHSP 70 expression was evaluated by qPCR analysis. Our results showed that BeWo trophoblastic cells treated with TgHSP 70 protein present lower levels of apoptosis even in the presence of inducers such as NaAsO2 and As2O3. The RH strain of T. gondii has a tendency to modulate apoptosis in BeWo cells compared to cells infected with ME 49 strain. Moreover, our qPCR results revelead that RH strain induces TgHSP70 expression in those cells. Finally, these data suggest that TgHSP70 can be directly associated to the decrease of apoptosis in BeWo cells. 2015-05-29T00:00:00Z https://repositorio.ufu.br/handle/123456789/47798 Title: Análise do perfil epidemiológico e clínico dos acidentes ofídicos registrados no Brasil na última década Abstract: Snakebite is a global public health problem, being considered a Neglected Tropical Disease by the World Health Organization. The dissemination of clinical and epidemiological information on snakebite accidents recorded in Brazil allows us to assess the severity of this public health problem in our country. Objective: To analyze the epidemiological and clinical profile of snakebites recorded in Brazil from 2012 to 2022. Methods: Descriptive and analytical review of epidemiological and clinical characteristics related to snakebites, based on data recorded in SINAN, from 2012 to 2022.The epidemiological analysis of snakebites was carried out based on data recorded in SINAN, from 2012 to 2022. The analysis of data relating to studies on snakebite poisoning found in the medical-scientific literature was carried out through a systematic review, using the PRISMA method. Results and Discussion: 277,394 snakebites were recorded in the country, with an average of 25,217.6 cases per year, of which 80% are caused by snakes of the genus Bothrops and occur predominantly in the North and Northeast regions. We observed a predominance of male victims (77%) and those of economically active age (predominantly in the age group of 20 to 39 years of age, with 34% of cases). These data corroborate the articles studied in this work, and with the literature in general. The epidemiological profile of snakebites recorded in Brazil has remained similar throughout this decade and data on the victims' clinical conditions suggest that, despite the considerable percentage of cases with moderate to severe symptoms, which can culminate in disabling sequelae and even in deaths, the majority of victims received rapid medical care and adequate treatment. The early use of specific antivenom serum for each genus of venomous snake, in the first hours after the victim is bitten by a snake, is decisive for adequate treatment, avoids complications and serious injuries caused by the venoms, and greatly increases the chances of a cure. Conclusion: Snakebites are a non-eradicable problem. Therefore, the union between science and public policies is very important to develop measures to prevent and control snakebite in Brazil. Such measures must consider regional specificities, such as: socioeconomic, educational and cultural level of the population, access to information and specialized medical centers. 2024-07-29T00:00:00Z