https://repositorio.ufu.br/handle/123456789/5495 2026-04-24T03:51:36Z 2026-04-24T03:51:36Z https://repositorio.ufu.br/handle/123456789/48441 2026-03-12T19:46:50Z 2026-02-19T00:00:00Z

Title: Plataforma Portátil de Detecção de MicroRNA Salivar Associado ao Câncer de Mama Utilizando Leitura Eletroquímica via Smartphone Abstract: This work reports the development of a portable electrochemical biosensor for the selective detection of salivary miR-200a, a key microRNA biomarker associated with breast cancer progression and epithelial–mesenchymal transition. The sensing platform was constructed using a screen-printed carbon electrode (SPCE) modified with an electropolymerized poly-PABA film, which provides a stable and functional interface enriched with carboxyl and amine groups for efficient probe immobilization while limiting nonspecific adsorption. A specific miR-200a oligonucleotide probe was immobilized onto the poly-PABA layer, and hybridization events were monitored through indirect electrochemical detection using ninhydrin as an electroactive indicator. The biosensor exhibited excellent analytical performance, including a wide linear response range, low limit of detection, good reproducibility, and operational stability for up to 30 days under refrigerated storage. Surface characterization by atomic force microscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy confirmed successful electrode functionalization and pronounced morphological changes following specific probe–target hybridization. High selectivity was demonstrated, as the complementary miR-200a target produced a significantly higher electrochemical response compared to non-complementary miRNA sequences and viral RNA (HCV), confirming minimal interference in complex 155 SERVIÇOPÚBLICOFEDERAL UNIVERSIDADEFEDERALDEUBERLÂNDIA INSTITUTO DEBIOTECNOLOGIA PROGRAMADEPÓS-GRADUAÇÃOEMGENÉTICAE BIOQUÍMICA biological matrices. Clinical validation using human saliva samples enabled clear discrimination between healthy individuals, patients with benign breast disease, breast cancer, and triple-negative breast cancer. Receiver operating characteristic (ROC) analysis yielded areas under the curve of 1.0 across all comparisons, indicating outstanding diagnostic accuracy. The integration of smartphone assisted electrochemical readout further enhances the portability and accessibility of the platform. Overall, this biosensor represents a promising non-invasive, low cost, and point-of-care diagnostic approach for breast cancer screening and molecular stratification.

2026-02-19T00:00:00Z https://repositorio.ufu.br/handle/123456789/47541 2025-11-01T06:22:07Z 2025-08-28T00:00:00Z

Title: Formulações de carreadores lipídicos nanoestruturados baseados em lipídios naturais com atividade anestésica Abstract: Pain is an unpleasant sensory and emotional experience associated with actual or potential tissue damage. It is considered a clinical condition when persisting for months or even years, as in chronic pain. Due to its long-term nature, chronic pain significantly compromises quality of life and mental health. The interaction of physical and emotional factors emphasizes the complexity of chronic pain and its management, requiring a multifactorial therapeutic approach. Local anesthetics (LAs) are widely used in the management of chronic pain. However, they present limitations such as short duration of action, systemic toxicity, and risk of dependence. Essential oils (EOs) have emerged as a promising alternative due to their biocompatibility and therapeutic properties, including analgesic and anesthetic effects. However, their physicochemical instability, toxicity, and high hydrophocity require strategies to enable their therapeutic use. Nanoencapsulation using nanostructured lipids carriers (NLCs) aims to overcome these challenges, improving delivery to biological tissues. In this study, 8 NLC formulations based on natural lipids, including anesthetic EOs, were developed. Long-term (365 days at 25 °C) and thermal physicochemical stability was monitored. The formulations with juniper (F3), lemongrass (F5), bergamot (F6), and melissa (F8) EOs exhibited the best structural properties after 1 year of monitoring. These formulations were evaluated for nanotoxicity and efficacy using an alternative biological model, Drosophila melanogaster (DM). Concentrations of 2.5, 1.25, and 0.625 mg/mL were safe, showing no toxicity. By the nociception test in DM larvae, F5 and F8 (2.5 mg/mL) approximately doubled and tripled the anesthesic effect compared to the untreated group, respectively. These systems were characterized by FTIR-ATR, DSC, and FE-SEM techniques, demonstrating compatibility among the excipients, thermal stability, and spherical morphology. Therefore, formulations F5 and F8 represent viable candidates to be further evaluated for safety and efficacy in more complex biological models, aiming to establish an alternative therapeutic option to the drugs traditionally used for pain management.

2025-08-28T00:00:00Z https://repositorio.ufu.br/handle/123456789/47481 2025-10-23T14:50:32Z 2024-01-31T00:00:00Z

Title: Filmes bio-híbridos magnéticos para entrega transdérmica de nanopartículas de ferrita de cobalto-gadolínio com atividade antineoplásica Abstract: Nanocomposites are promising smart drug delivery systems for cancer treatment. This work describes the innovative process of obtaining magnetic sodium alginate films with cobalt-gadolinium ferrite nanoparticles, for the transdermal melanoma treatment. Gadolinium-incorporated cobalt ferrite nanoparticles, exhibiting ferromagnetic behavior, were synthesized using the coprecipitation method. The magnetic nanoparticles were incorporated into the polymeric matrix, distributed over the entire surface. The magnetic biohybrid films showed homogeneity, flexibility, grayish color and thickness around 0.0659 μm, indicating the reproducibility of the preparation method. Magnetic saturation (Ms) was 0.22 emu/g and coercivity (Hc) 1.18 kOe. The incorporation of gadolinium ions influenced the rigidity of the membranes without compromising the magnetic capacity. The films showed significant cytotoxic activity against murine melanoma cells in in vitro tests. The in vivo nanotoxicity assay on a Drosophila melanogaster model determined that the prepared systems were biocompatible.

2024-01-31T00:00:00Z https://repositorio.ufu.br/handle/123456789/46760 2025-09-06T06:18:32Z 2025-08-04T00:00:00Z

Title: Influência de novos complexos ternários de cobre sobre o perfil transcricional de lncRNAs em células de câncer de mama triplo-negativo Abstract: Breast cancer (BC) is the most frequently diagnosed malignant tumor in women worldwide. Among its subtypes, triple-negative BC (TNBC) stands out for its aggressiveness and resistance to conventional treatments, as it lacks the expression of estrogen (ER) and progesterone (PR) hormone receptors, as well as the human epidermal growth factor receptor type 2 (HER2). Recently, copper(II)-Cu(II) complexes have emerged as promising antitumor agents, and, in the present study, four novel complexes were evaluated for their biological effects on BC cell lines. Compounds 2 and 3 showed high cytotoxicity and selectivity, inhibiting the clonogenicity and migration of TNBC cells, in addition to the activation of caspases 3 and 8. Interestingly, 2 and 3 negatively modulated the expression of lncRNAs associated with epithelial-mesenchymal transition, suggesting that these complexes act on multiple mechanisms related to tumor aggressiveness. These findings indicate that complexes 2 and 3 have promising therapeutic potential for the treatment of TNBC, reinforcing the need to further explore the molecular mechanisms involved in their antitumor activity.

2025-08-04T00:00:00Z