https://repositorio.ufu.br/handle/123456789/5162 2026-07-23T20:38:30Z 2026-07-23T20:38:30Z https://repositorio.ufu.br/handle/123456789/49010 2026-07-23T17:31:02Z 2026-07-03T00:00:00Z

Title: Memorial Descritivo

2026-07-03T00:00:00Z https://repositorio.ufu.br/handle/123456789/48861 2026-07-16T06:27:01Z 2025-04-29T00:00:00Z

Title: Efeitos da expressão de proteínas de SARS-CoV-2 no cérebro de Drosophila melanogaster

2025-04-29T00:00:00Z https://repositorio.ufu.br/handle/123456789/48852 2026-07-15T06:26:00Z 2026-04-24T00:00:00Z

Title: Perfil de quimiocinas e citocinas séricas e padrões fenotípicos de linfócitos T e B de memória ao longo do envelhecimento humano Abstract: Population aging represents one of the most significant contemporary demographic transformations, driven by increased life expectancy and declining fertility rates, with direct implications for the organization and functionality of the immune system. In this context, this PhD thesis is derived from the integration of three complementary studies investigating immune remodeling across the life course from an immunobiographical perspective. The first study, conducted in older adults, demonstrated a rhythmic increase in circulating inflammatory mediators during aging, particularly CXCL9, CXCL10, IL-1β, IL-6, and TNF, accompanied by a reduction in global connectivity among mediators and a reorganization of inflammatory networks, with increased functional centrality of these molecules. The second study, focused on childhood and adolescence, identified a bimodal pattern in memory cell dynamics, with an initial predominance of naïve cells around 2 years of age, followed by a more pronounced expansion of memory phenotypes between 8–10 years, alongside a progressive increase in connectivity among cellular subsets, especially during adolescence. The third study, encompassing healthy individuals aged 9 months to 65 years, demonstrated that T- and B-cell memory remodeling occurs in discrete, sex-dependent waves, with the most pronounced changes observed at 5–6 years, 10–19 years, and 20–29 years; women exhibited an earlier onset of these changes, whereas men showed greater variability and expansion of differentiated subsets, including effector and interferon-mediated phenotypes. Collectively, these findings indicate that immune remodeling across the lifespan follows dynamic and interconnected patterns involving both cellular compartments and soluble mediators, reflecting cumulative processes shaped by immunobiography. These trajectories, likely modulated by antigenic exposures, environmental factors, and physiological transitions, influence the balance between trained immunity and inflammatory differentiation, ultimately contributing to interindividual variability in immune resilience and susceptibility to chronic inflammatory conditions during aging.

2026-04-24T00:00:00Z https://repositorio.ufu.br/handle/123456789/48810 2026-07-11T06:18:40Z 2026-03-20T00:00:00Z

Title: Infecção placentária por Trypanosoma cruzi: papel da proteína P21 e avaliação do potencial terapêutico da óleo-resina e extrato hidroalcoólico das folhas de Copaifera multijuga Abstract: Congenital Chagas disease (CCD), caused by the transplacental transmission of Trypanosoma cruzi, can lead to severe fetal complications. However, the mechanisms of placental barrier transposition and the parasite molecules involved remain poorly understood. Among these molecules, the P21 protein stands out for mediating cellular invasion and proliferation, in addition to exhibiting diverse biological activities. Given the limitations of conventional treatment with benznidazole and nifurtimox, compounds from the Copaifera genus emerge as promising therapeutic alternatives. This study aimed to fulfill two general objectives: (I) to evaluate the effects of the oleoresin (OR) and the leaf hydroalcoholic extract (LHE) of Copaifera multijuga on T. cruzi infection in trophoblastic cells (BeWo) and third-trimester human placental explants; and (II) to investigate the role of P21 in this infection using BeWo cells and both first- and third-trimester placental explants. Regarding the first objective, both compounds reduced invasion, proliferation, and trypomastigote release in BeWo cells and placental explants, altering parasite morphology and modulating the immune response (cytokines and reactive oxygen species). This demonstrates the potential of C. multijuga in controlling infection at the maternal-fetal interface. Regarding the second objective, the absence of P21 (p21-/-) reduced the parasite's invasive capacity in BeWo cells, although it increased its initial proliferation. In placental explants, however, the lack of P21 impaired T. cruzi proliferation, retaining it within the syncytiotrophoblast and altering the local inflammatory profile (particularly in the first trimester), without preventing the infection-induced collagen reduction in the third trimester. In conclusion, the P21 protein is essential for the establishment and modulation of T. cruzi infection at the maternal-fetal interface.

2026-03-20T00:00:00Z