https://repositorio.ufu.br/handle/123456789/5162 2026-08-13T03:09:25Z 2026-08-13T03:09:25Z https://repositorio.ufu.br/handle/123456789/49474 2026-08-12T18:21:02Z 2026-04-24T00:00:00Z

Title: Perfil clínico, laboratorial e transfusional de pacientes oncológicos ambulatoriais: análise de três anos em hospital público brasileiro Abstract: Cancer represents a major public health concern and is frequently associated with hematological alterations resulting from both the disease itself and the therapies employed. In this context, transfusion support and the evaluation of laboratory parameters play a central role in the clinical management of these patients. This study aimed to characterize the clinical, laboratory, and transfusional profile of outpatient cancer patients treated at a public hospital in Brazil. This was a retrospective observational study including 389 patients treated between January 2020 and December 2022. The population had a median age of 62 years, with a slight predominance of females (53%). Solid tumors accounted for 52.9% of cases, followed by lymphoid (31.4%) and myeloid neoplasms (15.4%). During the study period, a total of 677 units of blood components were transfused, predominantly red blood cells concentrates (81.4%), with a median of two units per patient. Anemia was the main indication for transfusion (74.1%). Laboratory evaluation revealed consistent hematological abnormalities, including increased red cell distribution width (RDW), suggesting significant erythrocyte morphological heterogeneity. Additionally, elevated neutrophil-to-lymphocyte ratio (NLR) and platelet-to- lymphocyte ratio (PLR) indicated the presence of systemic inflammation. In the transfusional analysis, patients with solid tumors showed a higher intensity of red blood cell transfusion, whereas those with hematological neoplasms exhibited a higher frequency of platelet transfusion. Notably, platelet transfusion intensity was higher in patients with solid tumors, while among hematological neoplasms, the lymphoid subgroup demonstrated a greater relative demand for this component. Irregular antibody positivity was identified in 4.1% of patients, with a predominance of alloantibodies from the Rh and Kell systems. The occurrence of transfusion reactions was low, with three events reported. In conclusion, outpatient cancer patients present a high transfusional demand, with distinct patterns according to the type of neoplasm, as well as laboratory findings consistent with systemic inflammation and erythropoietic dysregulation. These findings highlight the importance of individualized transfusion strategies and contribute to improving clinical care in this population.

2026-04-24T00:00:00Z https://repositorio.ufu.br/handle/123456789/49440 2026-08-12T06:28:48Z 2026-07-31T00:00:00Z

Title: Desenvolvimento de estratégias antivirais frente a vírus emergentes Abstract: Emerging viruses pose growing threats to public health, particularly in Brazil, where environmental and sociodemographic factors favour epidemic outbreaks. This work aimed to identify and characterise antiviral compounds against the emerging viruses Orthobunyavirus oropoucheense (OROV) and Betacoronavirus pandemicum (SARS-CoV-2) through medium-throughput screening of the Pandemic Response Box (PRB) and Global Health Priority Box (GHPB) libraries from the Medicines for Malaria Venture (MMV). OROV is the causative agent of Oropouche fever, which caused over 13,000 cases in Brazil in 2024, with unprecedented geographical expansion, vertical transmission, neurological complications and confirmed fatalities, with no approved antiviral treatment available. SARS-CoV-2, responsible for the COVID-19 pandemic with over 779 million cases recorded through 2026, remains a therapeutic challenge, particularly against the Delta and Omicron variants of concern. The experimental approach employed fluorescent reporter viruses, BUNV-eGFP (Bunyamwera virus with an enhanced green fluorescent protein reporter) as a prototypic model for OROV, and SARS-CoV-2-mCherry (SARS-CoV-2 with a monomeric red fluorescent protein reporter) for direct screening, in A549 and A549-AT cells, with automated readout using the IncuCyte S3 system. Of the 640 compounds tested, active hits were identified and validated for both viral targets. Against OROV, nine compounds from the PRB inhibited BUNV replication by more than 90%. Following validation against wild-type OROV (epidemic strain AM0088), three hits were confirmed: Trimetrexate, MMV1634385, and GSK-983, being GSK the compound with greatest inhibition (EC50 = 0.5 μM against OROV; DHODH inhibitor; SI > 20), reported here for the first time as an anti-orthobunyavirus compound and identified as the most promising candidate. Against SARS-CoV-2, two hits were mechanistically characterised. Revaprazan (EC50 = 1.3 μM; SI > 7.7), a clinically approved potassium-competitive acid blocker (P-CAB), inhibited viral entry while retaining activity against Delta and Omicron BA.2, representing the first report of antiviral activity for a P-CAB-class compound. Milbemectin (EC50 = 1.6 μM; SI > 6), a milbemycin-class macrolide antiparasitic structurally related to ivermectin, acted predominantly at post-entry stages, interfering with virion assembly and egress. Together, these findings establish GSK-983, Revaprazan and Milbemectin as priority candidates for preclinical investigation, expand the antiviral spectrum of compounds with established safety profiles, and validate the fluorescent reporter-based screening platform as a robust strategy for antiviral discovery against emerging viral threats.

2026-07-31T00:00:00Z https://repositorio.ufu.br/handle/123456789/49419 2026-08-12T06:27:35Z 2025-12-12T00:00:00Z

Title: Análise in silico e in vitro da interação da BthTX-I, uma PLA2 Lys-49 da peçonha de Bothrops jararacussu, com proteínas-alvo associadas à infecção por Toxoplasma gondii

2025-12-12T00:00:00Z https://repositorio.ufu.br/handle/123456789/49418 2026-08-12T06:28:47Z 2026-03-26T00:00:00Z

Title: Pesquisa do agente da Febre Maculosa Rickettsia parkeri cepa Mata Atlântica no Estado de Minas Gerais Abstract: Ticks are hematophagous ectoparasites responsible for transmitting diseases to humans and an-imals. Among these diseases, Brazilian Spotted Fever, caused by Rickettsia rickettsii, stands out due to its high lethality when diagnosed late. Confirmed cases of this disease are closely associated with ticks of the genus Amblyomma, which represents the greatest diversity of ixodid species in Brazil. On the other hand, other species of the genus Rickettsia, such as Rickettsia parkeri Atlantic rainforest strain (ARF), may cause milder and frequently underdiagnosed clin-ical manifestations, although they still hold epidemiological importance. Minas Gerais is a state of large geographic proportions, with areas that favor the circulation of both bacteria, as well as their vectors. However, R. parkeri ARF , associated with its main vector Amblyomma ovale, had never been reported in this state. In this study, divided into two articles, we evaluated in the state of Minas Gerais the exposure of dogs, important sentinels of this disease, to the rick-ettsial antigens, as well as the presence of genetic material in ticks, in addition to assessing the seasonality of A. ovale, the main vector of R. parkeri ARF. These observations allowed us to conclude that the vector A. ovale shows higher frequency and abundance in spring, with greater intensity in November. Furthermore, the results provided evidence of exposure to rickettsial antigens in dogs from rural properties in the state of Minas Gerais, Brazil. Furthermore, the presence of R. parkeri ARF DNA was detected in two A. ovale ticks, constituting the first report of this bacterium associated with this tick in Minas Gerais.

2026-03-26T00:00:00Z