https://repositorio.ufu.br/handle/123456789/21238 2026-08-10T06:19:28Z 2026-08-10T06:19:28Z https://repositorio.ufu.br/handle/123456789/49366 2026-08-08T06:22:58Z 2026-03-13T00:00:00Z

Title: Avaliação in vitro de metabólitos termoestáveis de Bacillus spp. no controle da Macrophomina phaseolina

2026-03-13T00:00:00Z https://repositorio.ufu.br/handle/123456789/49325 2026-08-07T06:22:32Z 2026-03-12T00:00:00Z

Title: Extratos vegetais de Anemopaegma arvense e Jacaranda decurrens: quantificação de compostos fenólicos, atividade antimicrobiana, atividade antioxidante e aplicação na síntese verde de nanopartículas Abstract: The biodiversity of the Brazilian Cerrado represents a promising source of bioactive compounds with potential biotechnological applications. Among the native species of this biome, Anemopaegma arvense, popularly known as catuaba, and Jacaranda decurrens, known as carobinha, were selected. Both belong to the Bignoniaceae family and have a history of use in traditional medicine. Furthermore, scientific interest in studying plant extracts as sustainable alternatives in the biosynthesis of nanoparticles has been increasing, as this approach reduces the use of toxic reagents and makes processes more environmentally viable. Crude extracts were obtained from different parts of Anemopaegma arvense (leaves, roots, and fruits) and from the leaves of Jacaranda decurrens through dynamic maceration assisted by ultrasound, aiming to optimize the extraction of bioactive compounds. The analysis revealed a significant presence of phenolic compounds in all extracts, with emphasis on the leaves of Jacaranda decurrens, which showed the highest concentration. Among the parts of Anemopaegma arvense, the leaves demonstrated the highest content of these compounds, followed by the roots and, lastly, the fruits. In antioxidant assays, both by the ABTS radical scavenging method and by reducing power, a dose-dependent behavior was observed, with increased activity as extract concentration increased. The leaf extract of Jacaranda decurrens showed the best antioxidant performance, while the leaves and roots of Anemopaegma arvense also demonstrated relevant activity. Regarding antimicrobial activity, no significant results were observed under the evaluated conditions. However, the biosynthesis of gold nanoparticles was successfully achieved, demonstrating the ability of the extracts to act as reducing and stabilizing agents. The results highlight the biotechnological value of Cerrado species, especially concerning antioxidant activity and their application in sustainable nanoparticle synthesis processes, in addition to contributing to the scientific knowledge and application of these native plants.

2026-03-12T00:00:00Z https://repositorio.ufu.br/handle/123456789/49103 2026-07-26T06:18:42Z 2026-03-09T00:00:00Z

Title: Investigação da Formação de Biofilmes in vitro pela Linhagem Acidobacteria AB23 Abstract: Biofilms are microbial aggregates enveloped in a primarily polysaccharide matrix, synthesized by one or more members of that biofilm. Pseudomonas and Staphylococcus species are known biofilm producers; however, newly discovered or less studied bacterial groups lack demonstrated biofilm-forming capabilities. Among them is the phylum Acidobacteriota, which are common soil bacteria but have few isolates in culture. The overall objective of this study was to explore the in vitro production of biofilms in the AB23 strain of Acidobacteriota. Qualitative and quantitative methods related to the production of extracellular structures associated with biofilm formation were performed, including capsule staining, Congo Red Agar (CRA), and the crystal violet assay in microtiter plates. The results demonstrated the presence of a capsule in the AB23 bacterial strain. Additionally, biofilm formation was observed in the control group: Staphylococcus epidermidis. The AB23 strain did not show growth in BHI medium, even at a more acidic pH. Assays confirmed the growth of the AB23 strain in VL-55 medium at pH 6.5, containing Congo red, showing a color change suggestive of biofilm formation. Finally, the data obtained through the microtiter plate method corroborate the previous data, suggesting the capacity of the AB23 strain to produce biofilms. This set of results suggests the capacity of the AB23 strain to form biofilms in vitro. Considering future prospects for this strain, a possible application in the area of bioremediation is envisioned.

2026-03-09T00:00:00Z https://repositorio.ufu.br/handle/123456789/49074 2026-07-25T06:27:46Z 2026-03-05T00:00:00Z

Title: Efeitos biológicos in vitro de extratos da espécie de Renealmia petasites em células de câncer de mama triplo negativo Abstract: Breast cancer remains a significant challenge for healthcare systems, especially due to the aggressiveness of the triple-negative subtype, which has mortality rates of 30 to 40% within five years of diagnosis. Although chemotherapy is the primary treatment for these tumors, problems such as tumor resistance limit its effectiveness. Thus, natural products emerge as promising, overcoming not only therapeutic failure but also associated toxicity. This study aimed to evaluate the effect of Renealmia petasites extracts in controlling breast tumor cells. To this end, the MDA-MB-231 (triple-negative breast cancer), MCF-7 (luminal breast cancer), MCF-10A (non-tumorigenic), and HFF (human fibroblast) cell lines were treated with the crude extract of the plant's dried leaf (EAT 25) and its fractions (EAT 39-EAT 44), and different biological responses were evaluated. Cytotoxicity, clonogenicity, migration, and qPCR assays were conducted to quantify IL 6, IL-1β, and TGF-β. The crude extract EAT25 showed a mean inhibitory concentration (IC50) of 123.1 µg/mL in the MDA-MB-231 cell line after 48 hours of treatment, while fractions EAT40 to 43 did not significantly reduce viability (IC50 > 500 µg/mL). Fractions EAT39 and EAT44, with IC50s of 24.81 µg/mL and 47.63 µg/mL and selectivity indices of 2.49 and 3.0, respectively, were used in subsequent experiments. A dose dependent reduction in the migration of the MDA-MB-231 cell line was observed after 24 hours of treatment with EAT 39 and EAT 44, with only EAT 44 inhibiting this behavior after 48 hours. Clonogenicity, also after 48 hours, was inhibited for both fractions. Both EAT 39 and EAT 44 reduced IL6 and IL1-β transcripts. Therefore, the species R.petasites shows promise in controlling triple-negative breast cancer cells, so the purification and characterization of the major components of EAT 39 and EAT 44 are essential for validating their antitumor effects, especially in animal models.

2026-03-05T00:00:00Z