DSpace Collection:https://repositorio.ufu.br/handle/123456789/191782026-08-08T10:53:14Z2026-08-08T10:53:14ZAvaliação in silico de potenciais inibidores peptídicos derivados do transcriptoma da peçonha de Odontomachus chelifer contra alvos relacionados à doença de Alzheimerhttps://repositorio.ufu.br/handle/123456789/492902026-08-05T06:21:24Z2026-07-10T00:00:00ZTitle: Avaliação in silico de potenciais inibidores peptídicos derivados do transcriptoma da peçonha de Odontomachus chelifer contra alvos relacionados à doença de Alzheimer2026-07-10T00:00:00ZTriagem do potencial antimicrobiano de nove bactérias isoladas do alimento larval de abelhas sem ferrão nativas do Brasilhttps://repositorio.ufu.br/handle/123456789/491852026-07-31T06:23:39Z2025-09-29T00:00:00ZTitle: Triagem do potencial antimicrobiano de nove bactérias isoladas do alimento larval de abelhas sem ferrão nativas do Brasil2025-09-29T00:00:00ZFrequência gênica e genotípica da β-caseína a1 e a2 nas diferentes raças bovinas leiteiras de Uberlândia – Minas Geraishttps://repositorio.ufu.br/handle/123456789/491152026-07-28T06:21:15Z2026-03-18T00:00:00ZTitle: Frequência gênica e genotípica da β-caseína a1 e a2 nas diferentes raças bovinas leiteiras de Uberlândia – Minas Gerais
Abstract: β-casein is a major protein in bovine milk and exists in two predominant genetic variants: A1 and A2. During the digestion of A1 β-casein, β-casomorphin-7 is released—a peptide associated with gastrointestinal discomfort and other potential adverse effects on human health—whereas the A2 variant does not produce this compound. Therefore, this study aimed to determine the gene and genotype frequencies of A1 and A2 β-casein in various dairy cattle breeds from the Triângulo Mineiro region. Hair follicle samples were analyzed from 113 cows belonging to the Holstein, Girolando, and Jersolando breeds, as well as half-blood animals. DNA was extracted and subjected to allele-specific PCR (AS-PCR) to identify A1A1, A1A2, and A2A2 genotypes, followed by agarose gel electrophoresis to visualize the amplified products. The results showed a predominance of the A2 allele across all evaluated categories, with an overall frequency of 69.91%, while the A1 allele showed a frequency of 30.09%. Regarding genotypes, frequencies of 5.31% for A1A1, 49.56% for A1A2, and 45.13% for A2A2 were observed. The Girolando breed exhibited the highest frequency of the A2 allele (83.3%). Furthermore, the high proportion of heterozygotes suggests that the herds are undergoing a genetic transition, likely influenced by selection aimed at A2 milk production. These results highlight the importance of genotyping as a tool for genetic improvement programs, contributing to the selection of A2A2 animals and the development of herds that are more competitive and aligned with consumer market demands.2026-03-18T00:00:00ZMétodo de maturação ovocitária e cultivo embrionário bovino sob agitação 3D (por inclinação) lentahttps://repositorio.ufu.br/handle/123456789/490822026-07-25T06:27:49Z2026-03-20T00:00:00ZTitle: Método de maturação ovocitária e cultivo embrionário bovino sob agitação 3D (por inclinação) lenta
Abstract: In vitro embryo production (IVEP) is a technique that has revolutionized the bovine reproduction niche by enabling the selection and dissemination of genetic material of interest to improve herd reproductive and zootechnical efficiency. However, some stages of this process, such as in vitro oocyte maturation (IVM) and in vitro embryo culture (IVC), still present limitations, resulting in low rates of marketable blastocysts. Therefore, this study investigated the use of the 3D slow agitation method by tilting as an alternative to mimic the physiological environment of the fallopian tube in order to increase the efficiency of IVP. To this end, the continuous agitation regime was evaluated in various study groups and compared to control groups to investigate the impact of this methodology on cleavage rates, embryonic development, and blastocyst formation. Cleavage rates did not show significant differences between protocols, indicating that mechanical stimulation during in vitro maturation does not interfere with the initial number of embryos produced. However, embryonic development and, consequently, blastocyst formation were negatively affected, demonstrating that in vitro culture is a more sensitive stage and susceptible to environmental variations. The results showed that slow 3D tilting agitation, although a promising alternative due to its simplicity, low cost, and ease of application, still requires adjustments to the mechanical stimulus employed.2026-03-20T00:00:00Z